GMDS Polyklonaler Antikörper

GMDS Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(5)

Kat-Nr. : 15442-1-AP

Synonyme

SDR3E1, GMD, GDP-mannose 4,6 dehydratase, GDP-D-mannose dehydratase, EC:4.2.1.47

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  WB analysis using 15442-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15442-1-AP (GMDS antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of rat spleen using 15442-1-AP

  rat spleen tissue were subjected to SDS PAGE followed by western blot with 15442-1-AP (GMDS Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 15442-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15442-1-AP (GMDS antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 15442-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15442-1-AP (GMDS antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human lymphoma using 15442-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma using 15442-1-AP (GMDS antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human lymphoma using 15442-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma using 15442-1-AP (GMDS antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human colon cancer using 15442-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15442-1-AP (GMDS antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 15442-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15442-1-AP (GMDS antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 15442-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15442-1-AP (GMDS antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 15442-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 15442-1-AP (GMDS antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pancreas cancer using 15442-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 15442-1-AP (GMDS antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pancreas cancer using 15442-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 15442-1-AP (GMDS antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 15442-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 15442-1-AP (GMDS antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 15442-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using GMDS antibody (15442-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maus-Pankreasgewebe, HeLa-Zellen, Ratten-Pankreasgewebe, Rattenmilzgewebe
• Erfolgreiche Detektion in IHC: humanes Pankreaskarzinomgewebe, humanes Kolonkarzinomgewebe, humanes Lungenkarzinomgewebe, humanes Lymphomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunhistochemie (IHC): IHC : 1:500-1:2000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 5 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Produktinformation

15442-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA GMDS und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: GMDS fusion protein Ag7319
• Vollständiger Name: GDP-mannose 4,6-dehydratase
• Berechnetes Molekulargewicht: 42 kDa
• Beobachtetes Molekulargewicht: 40-42 kDa
• GenBank-Zugangsnummer: BC000117
• Gene symbol: GMDS
• Gene ID (NCBI): 2762
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

GDP-D-mannose-4,6-dehydratase (GMDS) is the key enzyme in the 'de novo' pathway of GDP-L-fucose biosynthesis(PMID:10462046). The enzyme is also named as GMD and belongs to the GDP-mannose 4,6-dehydratase family. The gene encodes a 42 kDa protein that catalyzes the conversion of GDP-mannose to GDP-4-keto-6-deoxymannose, the first step in the synthesis of GDP-fucose from GDP-mannose, using NADP+ as a cofactor.

Publikationen

Species	Application	Title
human	WB,IP	Mol Cell Biol; GDP-mannose-4,6-dehydratase is a cytosolic partner of tankyrase 1 that inhibits its poly(ADP-ribose) polymerase activity.; Authors - Bisht Kamlesh K KK
human	WB	Mol Cell Proteomics; Glycomic Profiling Highlights Increased Fucosylation in Pseudomyxoma Peritonei.; Authors - Lilli Saarinen
human	WB	Mol Cell Proteomics; Glycomic Profiling Highlights Increased Fucosylation in Pseudomyxoma Peritonei; Authors - Lilli Saarinen
human	WB	Cell Chem Biol; High-Sensitivity and Low-Toxicity Fucose Probe for Glycan Imaging and Biomarker Discovery.; Authors - Yasuhiko Kizuka
human	WB,IF	Stem Cell Reports; Low fucosylation defines the glycocalyx of progenitor cells and melanocytes in the human limbal stem cell niche; Authors - Ashley M Woodward