GMEB1-Specific Rekombinanter Antikörper

• Featured Product
• KD/KO Validated

GMEB1-Specific Rekombinant Antikörper für WB, FC (Intra), ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, FC (Intra), ELISA

Konjugation

Unkonjugiert

CloneNo.

241447C6

Kat-Nr. : 84448-1-RR

Synonyme

GMEB1, 241447C6, DNA-binding protein p96PIF, Glucocorticoid modulatory element-binding protein 1, GMEB 1

---

• 

  WB analysis of HepG2 using 84448-1-RR

  WB result of GMEB1-Specific antibody (84448-1-RR; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-GMEB1-Specific transfected HepG2 cells.

• 

  WB analysis using 84448-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84448-1-RR (GMEB1-Specific antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  FC experiment of HeLa using 84448-1-RR

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug GMEB1-Specific Recombinant antibody (84448-1-RR, Clone:241447C6) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, A549-Zellen, Daudi-Zellen, HepG2-Zellen
• Erfolgreiche Detektion in FC (Intra): HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

84448-1-RR bindet in WB, FC (Intra), ELISA GMEB1-Specific und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: glucocorticoid modulatory element binding protein 1
• Berechnetes Molekulargewicht: 63 kDa
• Beobachtetes Molekulargewicht: 88 kDa
• GenBank-Zugangsnummer: NM_006582
• Gene symbol: GMEB1
• Gene ID (NCBI): 10691
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

GMEB1 is a trans-acting factor that binds to glucocorticoid modulatory elements (GME), acting in estrogen receptor-regulated pathway GREB1 is expressed in proliferating prostate tissue & prostate cancer and is thought to function in hormone-dependent cancer growth. It associates with GMEB2 protein, and the complex is essential for parvovirus DNA replication.