GMEB2 Monoklonaler Antikörper

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GMEB2 Monoklonal Antikörper für WB, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

2H10H8

Kat-Nr. : 68010-1-PBS

Synonyme

DNA binding protein p79PIF, GMEB 2, GMEB2, KIAA1269, P79PIF, PIF p79, PIF79

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  WB analysis using 68010-1-Ig (same clone as 68010-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 68010-1-Ig (GMEB2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control. This data was developed using the same antibody clone with 68010-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 68010-1-Ig (same clone as 68010-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68010-1-Ig (GMEB2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control. This data was developed using the same antibody clone with 68010-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 68010-1-Ig (same clone as 68010-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68010-1-Ig (GMEB2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68010-1-PBS in a different storage buffer formulation.

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  WB analysis of MCF-7 using 68010-1-Ig (same clone as 68010-1-PBS)

  WB result of GMEB2 antibody (68010-1-Ig; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GMEB2 transfected MCF-7 cells. This data was developed using the same antibody clone with 68010-1-PBS in a different storage buffer formulation.

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  WB analysis of MCF-7 using 68010-1-Ig (same clone as 68010-1-PBS)

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 68010-1-Ig (GMEB2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68010-1-PBS in a different storage buffer formulation.

• 

  WB analysis of RAW 264.7 using 68010-1-Ig (same clone as 68010-1-PBS)

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 68010-1-Ig (GMEB2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68010-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

68010-1-PBS bindet in WB, Indirect ELISA GMEB2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: GMEB2 fusion protein Ag31393
• Vollständiger Name: glucocorticoid modulatory element binding protein 2
• Berechnetes Molekulargewicht: 530 aa, 56 kDa
• Beobachtetes Molekulargewicht: 68 kDa
• GenBank-Zugangsnummer: BC036305
• Gene symbol: GMEB2
• Gene ID (NCBI): 26205
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

GMEB-2 (glucocorticoid modulatory element-binding protein 2), also known as PIF79 (Parvovirus initiation factor p79) or P79PIF, is a DNA-binding protein that plays a crucial role in modulating transcription upon activation by steroid hormones. GMEB-2 is ubiquitously expressed with preferential expression in developmentally important tissues.