GMF Beta Monoklonaler Antikörper

GMF Beta Monoklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

3B10A2

Kat-Nr. : 60062-1-PBS

Synonyme

glia maturation factor beta, GMF Beta, GMFB, GMF-beta

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  WB analysis of fetal human brain using 60062-1-Ig (same clone as 60062-1-PBS)

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 60062-1-Ig (GMF-beta antibody at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60062-1-PBS in a different storage buffer formulation.

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  WB analysis of pig liver using 60062-1-Ig (same clone as 60062-1-PBS)

  pig liver tissue were subjected to SDS PAGE followed by western blot with 60062-1-Ig (GMF-beta antibody at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60062-1-PBS in a different storage buffer formulation.

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  IHC staining of human brain using 60062-1-Ig (same clone as 60062-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 60062-1-Ig (GMF-beta antibody at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 60062-1-PBS in a different storage buffer formulation.

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  IHC staining of human brain using 60062-1-Ig (same clone as 60062-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 60062-1-Ig (GMF-beta antibody at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 60062-1-PBS in a different storage buffer formulation.

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  IF Staining of U-87 MG using 60062-1-Ig (same clone as 60062-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed U-87 MG cells using GMF Beta antibody (60062-1-Ig, Clone: 3B10A2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60062-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 60062-1-Ig (same clone as 60062-1-PBS)

  Immunofluorescent analysis of Hela cells, using GMFB antibody 60062-1-Ig at 1:25 dilution and Rhodamine-labeled goat anti-mouse IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye). This data was developed using the same antibody clone with 60062-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

60062-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA GMF Beta und zeigt Reaktivität mit Hausschwein, human

• Getestete Reaktivität: Hausschwein, human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: GMF Beta fusion protein Ag1104
• Vollständiger Name: glia maturation factor, beta
• Berechnetes Molekulargewicht: 17 kDa
• Beobachtetes Molekulargewicht: 17 kDa
• GenBank-Zugangsnummer: BC005359
• Gene symbol: GMFB
• Gene ID (NCBI): 2764
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

GMFB, Glia maturation factor beta, is a nerve growth factor implicated in nervous system development. It was reported that GMF-beta promotes the phenotypic expression of glia and neurons and inhibits the proliferation of their respective tumors in culture cells. GMF-beta protein is specific to the brain where it is expressed in glial cells (mainly astrocytes) and insome neurons. Higher levels of GMFB were found in the central nervous system, except for the spinal cord, and in thymus and colon.