GMF Beta Polyklonaler Antikörper

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• KD/KO Validated

GMF Beta Polyklonal Antikörper für FC, IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IHC, IF, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

9

Kat-Nr. : 10690-1-AP

Synonyme

Glia maturation factor beta, glia maturation factor, beta, GMF, GMF beta, GMFB, GMF-β

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Galerie der Validierungsdaten

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  WB analysis using 10690-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10690-1-AP (GMF Beta antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis using 10690-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10690-1-AP (GMF Beta antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse heart using 10690-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 10690-1-AP (GMF-beta antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human heart using 10690-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 10690-1-AP (GMF-beta antibody) at dilution of 1:30 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 10690-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 10690-1-AP (GMF-beta antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 10690-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10690-1-AP (GMF-beta antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 10690-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10690-1-AP (GMF-beta antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse brain using 10690-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using 10690-1-AP (GMF-beta antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  FC experiment of U-87 MG using 10690-1-AP

  1X10^6 U-87 MG cells were intracellularly stained with 0.4 ug Anti-Human GMF Beta (10690-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP, Clone: ) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, humanes Herzgewebe, humanes Hirngewebe, Mausherzgewebe, PC-12-Zellen, Rattenhirngewebe, SH-SY5Y-Zellen, U-87 MG-Zellen
• Erfolgreiche Detektion in IHC: Maushirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: Maushirngewebe
• Erfolgreiche Detektion in FC: U-87 MG-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:50-1:500
• Durchflusszytometrie (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 7 publications below
• IHC: See 2 publications below
• IF: See 5 publications below

Produktinformation

10690-1-AP bindet in WB, IHC, IF, FC, ELISA GMF Beta und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Zebrafisch
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: GMF Beta fusion protein Ag1104
• Vollständiger Name: glia maturation factor, beta
• Berechnetes Molekulargewicht: 17 kDa
• Beobachtetes Molekulargewicht: 17 kDa
• GenBank-Zugangsnummer: BC005359
• Gene symbol: GMFB
• Gene ID (NCBI): 2764
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

GMFB, Glia maturation factor beta, is a nerve growth factor implicated in nervous system development. It was reported that GMF-beta promotes the phenotypic expression of glia and neurons and inhibits the proliferation of their respective tumors in culture cells. GMF-beta protein is specific to the brain where it is expressed in glial cells (mainly astrocytes) and insome neurons. Higher levels of GMFB were found in the central nervous system, except for the spinal cord, and in thymus and colon. GMFB was positive in the cytoplasm and nuclei of all cells in whole brain.

Publikationen

Species	Application	Title
mouse	IHC	Development; Coronary veins determine the pattern of sympathetic innervation in the developing heart.; Authors - Nam Joseph J
human	WB,IHC	Eur J Cancer; Identification of glia maturation factor beta as an independent prognostic predictor for serous ovarian cancer.; Authors - Li Yan Li YL
human	WB	Front Oncol; Glia Maturation Factor Beta as a Novel Biomarker and Therapeutic Target for Hepatocellular Carcinoma.; Authors - Wan Sun
human	WB,IF	Front Oncol; Glia Maturation Factor β as a Novel Independent Prognostic Biomarker and Potential Therapeutic Target of Kidney Renal Clear Cell Carcinoma.; Authors - Tong Zhu
mouse,zebrafish	WB,IF	Exp Neurol; Glia maturation factor beta is required for reactive gliosis after traumatic brain injury in zebrafish.; Authors - Guo Yin ; KO Validated
human	IF	J Alzheimers Dis; Co-Localization of Glia Maturation Factor with NLRP3 Inflammasome and Autophagosome Markers in Human Alzheimer's Disease Brain.; Authors - Mohammad Ejaz Ahmed