GMFG Polyklonaler Antikörper

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GMFG Polyklonal Antikörper für WB, IHC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 13625-1-PBS

Synonyme

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• 

  WB analysis of rat brain using 13625-1-AP (same clone as 13625-1-PBS)

  rat brain tissue were subjected to SDS PAGE followed by western blot with 13625-1-AP (GMFG Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13625-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human placenta using 13625-1-AP (same clone as 13625-1-PBS)

  human placenta tissue were subjected to SDS PAGE followed by western blot with 13625-1-AP (GMFG Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13625-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse placenta using 13625-1-AP (same clone as 13625-1-PBS)

  mouse placenta tissue were subjected to SDS PAGE followed by western blot with 13625-1-AP (GMFG Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13625-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human heart using 13625-1-AP (same clone as 13625-1-PBS)

  human heart tissue were subjected to SDS PAGE followed by western blot with 13625-1-AP (GMFG antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13625-1-PBS in a different storage buffer formulation.

• 

  IP experiment of mouse spleen using 13625-1-AP (same clone as 13625-1-PBS)

  IP result of anti-GMFG (IP:13625-1-AP, 4ug; Detection:13625-1-AP 1:500) with mouse spleen tissue lysate 4000ug. This data was developed using the same antibody clone with 13625-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 13625-1-AP (same clone as 13625-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 13625-1-AP (GMFG antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13625-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 13625-1-AP (same clone as 13625-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 13625-1-AP (GMFG antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13625-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 13625-1-AP (same clone as 13625-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney using 13625-1-AP (GMFG antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 13625-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 13625-1-AP (same clone as 13625-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney using 13625-1-AP (GMFG antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 13625-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human placenta using 13625-1-AP (same clone as 13625-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta using 13625-1-AP (GMFG antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 13625-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human placenta using 13625-1-AP (same clone as 13625-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta using 13625-1-AP (GMFG antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 13625-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human testis using 13625-1-AP (same clone as 13625-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis using 13625-1-AP (GMFG antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 13625-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human testis using 13625-1-AP (same clone as 13625-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis using 13625-1-AP (GMFG antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 13625-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human spleen using 13625-1-AP (same clone as 13625-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human spleen using 13625-1-AP (GMFG antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 13625-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human spleen using 13625-1-AP (same clone as 13625-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human spleen using 13625-1-AP (GMFG antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 13625-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human heart using 13625-1-AP (same clone as 13625-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 13625-1-AP (GMFG Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 13625-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human heart using 13625-1-AP (same clone as 13625-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 13625-1-AP (GMFG Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 13625-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

13625-1-PBS bindet in WB, IHC, IP, Indirect ELISA GMFG und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: GMFG fusion protein Ag4538
• Vollständiger Name: glia maturation factor, gamma
• Berechnetes Molekulargewicht: 141 aa, 17 kDa
• Beobachtetes Molekulargewicht: 17 kDa
• GenBank-Zugangsnummer: BC032819
• Gene symbol: GMFG
• Gene ID (NCBI): 9535
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

GMFG, is a highly conserved brain-specific protein that belongs to the GMF subfamily of the larger Actin-binding protein ADF family. GMFG is preferentially expressed in blood vessel cells and immune cells and its ectopic expression enhances cellular functions such as tube-formation and migration in vitro. GMFG mediates neutrophil and T-lymphocyte migration via regulation of actin cytoskeletal reorganization. GMFG also acts as an intracellular regulator of stress-activated signal transduction by demonstrating activation of p38 MAP kinase and transcription factor NF-kB in astrocytes.