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GMIP Rekombinanter Antikörper

GMIP Rekombinant Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

250483G2

Kat-Nr. : 86101-2-PBS

Synonyme

ARHGAP46, GEM interacting protein, GEM-interacting protein

Filter:
  • Western Blot (WB) analysis of various lysates using GMIP Recombinant monoclonal antibody (86101-2-RR)

    WB analysis using 86101-2-RR (same clone as 86101-2-PBS)

    Jurkat cell lysates were subjected to SDS PAGE followed by western blot with 86101-2-RR (GMIP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86101-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using GMIP Recombinant monoclonal antibody (86101-2-RR)

    IHC staining of human ovary cancer using 86101-2-RR (same clone as 86101-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 86101-2-RR (GMIP antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86101-2-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of K-562 cells using GMIP Recombinant monoclonal antibody (86101-2-RR)

    IF Staining of K-562 using 86101-2-RR (same clone as 86101-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed K-562 cells using GMIP antibody (86101-2-RR, Clone: 250483G2 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 86101-2-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 86101-2-RR

    Affinity and Kinetic Characterization of 86101-2-RR

    Biolayer interferometry (BLl) kinetic assays of 86101-2-RR against Human GMIP were performed. The affinity constant is 2.02 nM.

Geprüfte Anwendungen

Produktinformation

86101-2-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA GMIP und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Kaninchen / IgG
Klonalität Rekombinant
Typ Antikörper
Immunogen GMIP fusion protein Ag23239
Vollständiger Name GEM interacting protein
Beobachtetes Molekulargewicht 130 kDa
GenBank-ZugangsnummerBC126436
Gene symbol GMIP
Gene ID (NCBI) 51291
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

GMIP (Gem-interacting protein) is a 970 amino acid protein that stimulates the GTPase activity of RhoA in vitro and in vivo. GMIP interacts with Gem through its N-terminus and has a Rho GTPase-activating protein domain at its C-terminus. The Rho family of GTP-binding proteins plays a role in the development of neuronal structure. GMIP is able to inhibit RhoA function, leading to Actin cytoskeletal reorganization in vivo.