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Geminin Rekombinanter Antikörper

Geminin Rekombinant Antikörper für IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

240835E6

Kat-Nr. : 84199-1-PBS

Synonyme

GMNN, MGORS6, Gem, 240835E6

Filter:
  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using Geminin Recombinant antibody (84199-1-RR)

    IF Staining of HepG2 using 84199-1-RR (same clone as 84199-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using GMNN antibody (84199-1-RR, Clone: 240835E6 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84199-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84199-1-RR

    Affinity and Kinetic Characterization of 84199-1-RR

    Biolayer interferometry (BLl) kinetic assays of 84199-1-RR against Human GMNN were performed. The affinity constant is 0.876 nM.

Geprüfte Anwendungen

Produktinformation

84199-1-PBS bindet in IF/ICC, Indirect ELISA Geminin und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Kaninchen / IgG
Klonalität Rekombinant
Typ Antikörper
Immunogen Geminin fusion protein Ag1076
Vollständiger Name geminin, DNA replication inhibitor
Berechnetes Molekulargewicht 24 kDa
GenBank-ZugangsnummerBC005185
Gene symbol GMNN
Gene ID (NCBI) 51053
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein A purfication
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

GMNN, also designated geminin, contains 212 amino acids and has a destruction box sequence (RRTLKVIQP). GMNN participates in ininhibiting DNA replication by preventing the incorporation of MCM complex into pre-replication complex (pre-RC). It is degraded during the metaphase-anaphase transition of cell cycle's mitotic phase, which permits replication in the succeeding cell cycle. GMNN has a broad sedimentation profile ranging from about 25 kDa to 90 kDa, with a major peak at 30 kDa. Scanning of the signals shows that discrete peaks corresponding to apparent mass of 42.5 kDa and 66 kDa are present. (PMID: 15313623)