GMPPB Polyklonaler Antikörper

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• KD/KO Validated

GMPPB Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(7)

Kat-Nr. : 15094-1-AP

Synonyme

Mannose-1-phosphate guanyltransferase beta, KIAA1851, GTP-mannose-1-phosphate guanylyltransferase beta, GDP-mannose pyrophosphorylase B, EC:2.7.7.13

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  WB analysis using 15094-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15094-1-AP (GMPPB antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 15094-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 15094-1-AP (GMPPB antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human ovary cancer using 15094-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 15094-1-AP (GMPPB antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HEK-293 using 15094-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using 15094-1-AP (GMPPB antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maus-Skelettmuskelgewebe, Maushirngewebe, Ratten-Skelettmuskelgewebe
• Erfolgreiche Detektion in IHC: human ovary cancer tissue; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HEK-293-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 7 publications below
• IHC: See 1 publications below
• IF: See 2 publications below

Produktinformation

15094-1-AP bindet in WB, IHC, IF/ICC, ELISA GMPPB und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Zebrafisch
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: GMPPB fusion protein Ag7168
• Vollständiger Name: GDP-mannose pyrophosphorylase B
• Berechnetes Molekulargewicht: 40 kDa
• Beobachtetes Molekulargewicht: 40-43 kDa
• GenBank-Zugangsnummer: BC001141
• Gene symbol: GMPPB
• Gene ID (NCBI): 29925
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

GMPPB is a cytoplasmic enzyme that catalyzes the formation of GDP-mannose, a key substrate for several glycosylation pathways, including O-mannosylation of α-DG. GMPPB facilitates the formation of the sugar donor GDP-mannose and pyrophosphate from mannose-1-phosphate and GTP, a very early step in the glycosylation pathway (PMID: 37834154, 27147698). GMPPB mutations correlate with several congenital diseases, including severe congenital muscular dystrophy (CMD) with abnormalities in the brain and eye (PMID: 26310427, 30684953). Western blot analysis detected GMPPB at an apparent molecular mass of 40 kDa.

Publikationen

Species	Application	Title
human	WB	iScience; A Trace Amount of Galactose, a Major Component of Milk Sugar, Allows Maturation of Glycoproteins during Sugar Starvation.; Authors - Norio Sasaoka
mouse	WB	Front Mol Biosci; Altered Glycosylation in the Aging Heart.; Authors - Patricia Franzka
zebrafish	WB	Mol Biomed; GMPPB-congenital disorders of glycosylation associate with decreased enzymatic activity of GMPPB.; Authors - Zhe Liu
human,mouse	WB	Front Nutr; Altered mannose metabolism in chronic stress and depression is rapidly reversed by vitamin B12; Authors - Patricia Franzka
human	WB,IHC	Int J Mol Sci; Silencing GMPPB Inhibits the Proliferation and Invasion of GBM via Hippo/MMP3 Pathways; Authors - Zi-Lu Huang ; KD Validated
mouse	WB,IF	Front Mol Neurosci; Consequences of GMPPB deficiency for neuromuscular development and maintenance; Authors - Mona K Schurig