GNA15 Polyklonaler Antikörper

GNA15 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 12078-1-AP

Synonyme

G alpha 15, G alpha 16, G protein subunit alpha 15, G protein subunit alpha 16, GNA15, GNA16

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Galerie der Validierungsdaten

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  WB analysis using 12078-1-AP

  MOLT-4 cells were subjected to SDS PAGE followed by western blot with 12078-1-AP (GNA15 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis using 12078-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 12078-1-AP (GNA15 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IHC staining of human small intestine using 12078-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 12078-1-AP (GNA15 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human small intestine using 12078-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 12078-1-AP (GNA15 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: MOLT-4-Zellen, Mausmilzgewebe
• Erfolgreiche Detektion in IHC: humanes Dünndarmgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

12078-1-AP bindet in WB, IHC, ELISA GNA15 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: GNA15 fusion protein Ag2738
• Vollständiger Name: guanine nucleotide binding protein (G protein), alpha 15 (Gq class)
• Berechnetes Molekulargewicht: 374 aa, 44 kDa
• Beobachtetes Molekulargewicht: 44 kDa
• GenBank-Zugangsnummer: BC013585
• Gene symbol: GNA15
• Gene ID (NCBI): 2769
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

GNA15, also known as GNA16, belongs to the G-alpha family and G(q) subfamily. The protein encoded by the GNA15 gene is the alpha subunit of the G protein, which participates in the regulation of cell proliferation and apoptosis. GNA15 is highly expressed in some cell types, such as hematopoietic stem cells and epithelial cells during specific stages of differentiation. GNA15 is known to play as an oncogene in several tumors, such as gastroenteropancreatic neuroendocrine neoplasia (GEP-NEN), liver cancer, pancreatic ductal adenocarcinoma and ovarian cancer (PMID: 34552875). The molecular weight of GNA15 is 44 kDa.