GNAI1 Polyklonaler Antikörper

GNAI1 Polyklonal Antikörper für FC, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

2

Kat-Nr. : 12617-1-AP

Synonyme

Gi, GNAI1

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Galerie der Validierungsdaten

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  WB analysis using 12617-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12617-1-AP (GNAI1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of fetal human brain using 12617-1-AP

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 12617-1-AP (GNAI1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of SH-SY5Y using 12617-1-AP

  IP result of anti-GNAI1 (IP:12617-1-AP, 4ug; Detection:12617-1-AP 1:1000) with SH-SY5Y cells lysate 800 ug.

• 

  IHC staining of human kidney using 12617-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 12617-1-AP (GNAI1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 12617-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 12617-1-AP (GNAI1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat kidney using 12617-1-AP

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 12617-1-AP (GNAI1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat kidney using 12617-1-AP

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 12617-1-AP (GNAI1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  FC experiment of SH-SY5Y using 12617-1-AP

  1X10^6 SH-SY5Y cells were intracellularly stained with 0.20 ug/test Anti-Human GNAI1 (12617-1-AP) (red) or 0.20 ug control antibody (blue) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000. Fixed with90% MeOH.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, fetales humanes Hirngewebe, Rattenhirngewebe
• Erfolgreiche IP: SH-SY5Y-Zellen
• Erfolgreiche Detektion in IHC: humanes Nierengewebe, Rattennierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in FC: SH-SY5Y-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:500-1:2000
• Durchflusszytometrie (FC): FC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below

Produktinformation

12617-1-AP bindet in WB, IP, IHC, FC, ELISA GNAI1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: GNAI1 fusion protein Ag3290
• Vollständiger Name: guanine nucleotide binding protein (G protein), alpha inhibiting activity polypeptide 1
• Berechnetes Molekulargewicht: 354 aa, 40 kDa
• Beobachtetes Molekulargewicht: 38-40 kDa
• GenBank-Zugangsnummer: BC026326
• Gene symbol: GNAI1
• Gene ID (NCBI): 2770
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The guanine nucleotide-binding protein G(i), α-1 subunit (GNAI1), also known as Gαi-1, belongs to the Gαi family. GNAI1 is an adenylate cyclase inhibitor that modulates cAMP. GNAI1 exerts inhibitory effects on cAMP production through its interaction with GPCR and the inactivation of adenyl cyclase (PMID: 35678269). GNAI1 has 2 isoforms with molecular weights of 35 and 40 kDa, respectively.

Publikationen

Species	Application	Title
mouse	WB	Drug Des Devel Ther; Network Pharmacology and Experimental Verification to Explore the Potential Mechanism of Yin-Huo-Tang for Lung Adenocarcinoma Recurrence.; Authors - Dianna Liu
human	WB	J Oncol; Characterization of the Newly Established Homoharringtonine- (HHT-) Resistant Cell Lines and Mechanisms of Resistance; Authors - Fenglin Li