GNAI2 Polyklonaler Antikörper

GNAI2 Polyklonal Antikörper für IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

5

Kat-Nr. : 11136-1-AP

Synonyme

GIP, GNAI2, GNAI2B, H_LUCA15.1, H_LUCA16.1

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Galerie der Validierungsdaten

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  WB analysis using 11136-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11136-1-AP (GNAI2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of fetal human brain using 11136-1-AP

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 11136-1-AP (GNAI2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SKOV-3 using 11136-1-AP

  SKOV-3 cells were subjected to SDS PAGE followed by western blot with 11136-1-AP (GNAI2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 11136-1-AP

  IP Result of anti-GNAI2 (IP:11136-1-AP, 4ug; Detection:11136-1-AP 1:500) with mouse brain tissue lysate 4000ug.

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  IHC staining of mouse brain using 11136-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11136-1-AP (GNAI2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 11136-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11136-1-AP (GNAI2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 11136-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 11136-1-AP (GNAI2 antibody) at dilution of 1:10 (under 25x lens).

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  IHC staining of human lung cancer using 11136-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 11136-1-AP (GNAI2 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human brain using 11136-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 11136-1-AP (GNAI2 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human brain using 11136-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 11136-1-AP (GNAI2 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of mouse ovary using 11136-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 11136-1-AP (GNAI2 antibody) at dilution of 1:50 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse ovary using 11136-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 11136-1-AP (GNAI2 antibody) at dilution of 1:50 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 11136-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 11136-1-AP (GNAI2 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human kidney using 11136-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 11136-1-AP (GNAI2 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human tonsillitis using 11136-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 11136-1-AP (GNAI2 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human tonsillitis using 11136-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 11136-1-AP (GNAI2 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HeLa using 11136-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using GNAI2 antibody (11136-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: fetales humanes Hirngewebe, Maushirngewebe, Rattenhirngewebe, SKOV-3-Zellen
• Erfolgreiche IP: Maushirngewebe
• Erfolgreiche Detektion in IHC: Maushirngewebe, humanes Hirngewebe, humanes Lungenkarzinomgewebe, humanes Nierengewebe, humanes Tonsillitisgewebe, Maus-Eierstockgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 4 publications below
• IF: See 1 publications below
• CoIP: See 1 publications below

Produktinformation

11136-1-AP bindet in WB, IP, IHC, IF, CoIP, ELISA GNAI2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: GNAI2 fusion protein Ag1597
• Vollständiger Name: guanine nucleotide binding protein (G protein), alpha inhibiting activity polypeptide 2
• Berechnetes Molekulargewicht: 41 kDa
• Beobachtetes Molekulargewicht: 35-40 kDa
• GenBank-Zugangsnummer: BC012138
• Gene symbol: GNAI2
• Gene ID (NCBI): 2771
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

GNAI2, also named as GNAI2B, belongs to the G-alpha family. G(i/o/t/z) subfamily. Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. The G(i) proteins are involved in hormonal regulation of adenylate cyclase: they inhibit the cyclase in response to beta-adrenergic stimuli. GNAI2 is 93% homolog to GNAI1, 94% to GNAI3, 85% to GNAT3, 82% to GNAT2.

Publikationen

Species	Application	Title
mouse	WB	Nat Commun; EMX2-GPR156-Gαi reverses hair cell orientation in mechanosensory epithelia.; Authors - Katie S Kindt
human	WB	Oncotarget; PMID: 24658464; Authors - Jie Li
mouse	IF	bioRxiv; Inhibitory G proteins play multiple roles to polarize sensory hair cell morphogenesis; Authors - Amandine Jarysta
mouse	WB	Adv Sci (Weinh); Targeting Squalene Epoxidase Confers Metabolic Vulnerability and Overcomes Chemoresistance in HNSCC; Authors - Xinyuan Zhao
human	WB,CoIP	Sci Adv; RGS12 polarizes the GPSM2-GNAI complex to organize and elongate stereocilia in sensory hair cells; Authors - Anil Akturk