GNAI2 Monoklonaler Antikörper

GNAI2 Monoklonal Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(1)

CloneNo.

3F6H5

Kat-Nr. : 67007-1-Ig

Synonyme

3F6H5, Adenylate cyclase-inhibiting G alpha protein, GIP, GNAI2B, Guanine nucleotide-binding protein G(i) subunit alpha-2

---

• 

  WB analysis using 67007-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67007-1-Ig (GNAI2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of U-937 using 67007-1-Ig

  U-937 cells were subjected to SDS PAGE followed by western blot with 67007-1-Ig (GNAI2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  IF Staining of A431 using 67007-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using 67007-1-Ig (GNAI2 antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HL-60-Zellen, C6-Zellen, RAW 264.7-Zellen, U-937-Zellen
• Erfolgreiche Detektion in IF/ICC: A431-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:6000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below

Produktinformation

67007-1-Ig bindet in WB, IF/ICC, ELISA GNAI2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: Kaninchen
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: GNAI2 fusion protein Ag28560
• Vollständiger Name: guanine nucleotide binding protein (G protein), alpha inhibiting activity polypeptide 2
• Berechnetes Molekulargewicht: 41 kDa
• Beobachtetes Molekulargewicht: 35-40 kDa
• GenBank-Zugangsnummer: BC012138
• Gene symbol: GNAI2
• Gene ID (NCBI): 2771
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

GNAI2, also named as GNAI2B, belongs to the G-alpha family. G(i/o/t/z) subfamily. Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. The G(i) proteins are involved in hormonal regulation of adenylate cyclase: they inhibit the cyclase in response to beta-adrenergic stimuli. GNAI2 is 93% homolog to GNAI1, 94% to GNAI3, 85% to GNAT3, 82% to GNAT2.

Publikationen

Species	Application	Title
rabbit	WB	Genes (Basel); GNAI2 Promotes Proliferation and Decreases Apoptosis in Rabbit Melanocytes.; Authors - Shuaishuai Hu