GNAI2 Monoklonaler Antikörper

GNAI2 Monoklonal Antikörper für IF/ICC

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

IF/ICC

Konjugation

CoraLite®594 Fluorescent Dye

CloneNo.

3F6H5

Kat-Nr. : CL594-67007

Synonyme

H_LUCA15.1, Guanine nucleotide-binding protein G(i) subunit alpha-2, GNAI2B, GIP, Adenylate cyclase-inhibiting G alpha protein

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  IF Staining of A431 using CL594-67007

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using CoraLite®594 GNAI2 antibody (CL594-67007, Clone: 3F6H5 ) at dilution of 1:200.

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  FC experiment of U-937 using CL594-67007

  1x10^6 U-937 cells were intracellularly stained with 0.4 ug CoraLite®594-conjugated GNAI2 Monoclonal antibody (CL594-67007, Clone:3F6H5)(red), or 0.4 ug CoraLite®594 Mouse IgG1 Isotype Control (1F8D3) (CL594-66360-1, Clone: 1F8D3) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF/ICC: A431-Zellen
• Erfolgreiche Detektion in FC (Intra): U-937-Zellen

Empfohlene Verdünnung

• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

CL594-67007 bindet in IF/ICC GNAI2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: GNAI2 fusion protein Ag28560
• Vollständiger Name: guanine nucleotide binding protein (G protein), alpha inhibiting activity polypeptide 2
• Berechnetes Molekulargewicht: 41 kDa
• Beobachtetes Molekulargewicht: 35-40 kDa
• GenBank-Zugangsnummer: BC012138
• Gene symbol: GNAI2
• Gene ID (NCBI): 2771
• Konjugation: CoraLite®594 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 588 nm / 604 nm
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

GNAI2, also named as GNAI2B, belongs to the G-alpha family. G(i/o/t/z) subfamily. Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. The G(i) proteins are involved in hormonal regulation of adenylate cyclase: they inhibit the cyclase in response to beta-adrenergic stimuli. GNAI2 is 93% homolog to GNAI1, 94% to GNAI3, 85% to GNAT3, 82% to GNAT2.