GNAO1 Polyklonaler Antikörper

GNAO1 Polyklonal Antikörper für IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

9

Kat-Nr. : 12635-1-AP

Synonyme

G ALPHA o, GNAO, GNAO1

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Galerie der Validierungsdaten

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  WB analysis using 12635-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12635-1-AP (GNAO1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 12635-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12635-1-AP (GNAO1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human liver using 12635-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 12635-1-AP (GNAO1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Y79 using 12635-1-AP

  Y79 cells were subjected to SDS PAGE followed by western blot with 12635-1-AP (GNAO1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 12635-1-AP

  IP Result of anti-GNAO1 (IP:12635-1-AP, 3ug; Detection:12635-1-AP 1:600) with mouse brain tissue lysate 3600ug.

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  IHC staining of human gliomas using 12635-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 12635-1-AP (GNAO1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human gliomas using 12635-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 12635-1-AP (GNAO1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human gliomas using 12635-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 12635-1-AP (GNAO1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human gliomas using 12635-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 12635-1-AP (GNAO1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, humanes Lebergewebe, Maushodengewebe, Rattenhirngewebe, Rattenhodengewebe, Y79-Zellen
• Erfolgreiche IP: Maushirngewebe
• Erfolgreiche Detektion in IHC: humanes Gliomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:16000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 4 publications below
• IHC: See 4 publications below
• IF: See 2 publications below

Produktinformation

12635-1-AP bindet in WB, IP, IHC, IF, ELISA GNAO1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: GNAO1 fusion protein Ag3330
• Vollständiger Name: guanine nucleotide binding protein (G protein), alpha activating activity polypeptide O
• Berechnetes Molekulargewicht: 302 aa, 35 kDa
• Beobachtetes Molekulargewicht: 40 kDa
• GenBank-Zugangsnummer: BC030027
• Gene symbol: GNAO1
• Gene ID (NCBI): 2775
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

GNAO1 gene encodes the α subunit of heterotrimeric guanine nucleotide-binding protein (Gi protein), a membrane protein widely expressed in the central nervous system involved in signal transduction (PMID: 30642806). GNAO1 belongs to the G-alpha family and G(i/o/t/z) subfamily. GNAO1 is highly expressed in brain and modulate neuronal excitability (PMID: 30838255). GNAO1 has 2 isoforms with the molecular mass of 40 kDa.

Publikationen

Species	Application	Title
human	WB	Blood; Identification of functional cooperative mutations of GNAO1 in human acute lymphoblastic leukemia.; Authors - Lili Song
human	WB	Neurochem Res; Efficacy of Hybrid Tetrahydrobenzo[d]thiazole Based Aryl Piperazines D-264 and D-301 at D₂ and D₃ Receptors.; Authors - Juan Zhen
human	WB	Gene; DNMT1 mediated promoter methylation of GNAO1 in hepatoma carcinoma cells.; Authors - Diannan Xu
human	IHC	Int J Mol Med; Overexpression of GNAO1 correlates with poor prognosis in patients with gastric cancer and plays a role in gastric cancer cell proliferation and apoptosis.; Authors - Zhen Liu
human	IHC	Biosci Rep; The down-regulation of GNAO1 and its promoting role in hepatocellular carcinoma.; Authors - Pei Xiaoyu X
human	IHC	J Healthc Eng; GNAO1 as a Novel Predictive Biomarker for Late Relapse in Hepatocellular Carcinoma.; Authors - Meiling Du