GNAO1 Monoklonaler Antikörper

GNAO1 Monoklonal Antikörper für WB, IHC, IF-P, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, Huhn, human, Kaninchen, Maus, Ratte

Anwendung

WB, IHC, IF-P, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

1B5G2

Kat-Nr. : 68027-1-PBS

Synonyme

DKFZp686O0962, G ALPHA o, GNAO, GNAO1

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  WB analysis using 68027-1-Ig (same clone as 68027-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68027-1-Ig (GNAO1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with Lamin B1 Monoclonal antibody (66095-1-Ig) as loading control. This data was developed using the same antibody clone with 68027-1-PBS in a different storage buffer formulation.

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  IHC staining of human gliomas using 68027-1-Ig (same clone as 68027-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 68027-1-Ig (GNAO1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68027-1-PBS in a different storage buffer formulation.

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  IHC staining of human gliomas using 68027-1-Ig (same clone as 68027-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 68027-1-Ig (GNAO1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68027-1-PBS in a different storage buffer formulation.

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  IF Staining of rat cerebellum using 68027-1-Ig (same clone as 68027-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed rat cerebellum tissue using GNAO1 antibody (68027-1-Ig, Clone: 1B5G2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68027-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

68027-1-PBS bindet in WB, IHC, IF-P, Indirect ELISA GNAO1 und zeigt Reaktivität mit Hausschwein, Huhn, human, Kaninchen, Maus, Ratten

• Getestete Reaktivität: Hausschwein, Huhn, human, Kaninchen, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: GNAO1 fusion protein Ag32045
• Vollständiger Name: guanine nucleotide binding protein (G protein), alpha activating activity polypeptide O
• Berechnetes Molekulargewicht: 302 aa, 35 kDa
• Beobachtetes Molekulargewicht: 40 kDa
• GenBank-Zugangsnummer: BC030027
• Gene symbol: GNAO1
• Gene ID (NCBI): 2775
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

GNAO1 gene encodes the α subunit of heterotrimeric guanine nucleotide-binding protein (Gi protein), a membrane protein widely expressed in the central nervous system involved in signal transduction (PMID: 30642806). GNAO1 belongs to the G-alpha family and G(i/o/t/z) subfamily. GNAO1 is highly expressed in brain and modulate neuronal excitability (PMID: 30838255). GNAO1 has 2 isoforms with the molecular mass of 40 kDa.