GNAQ Polyklonaler Antikörper

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• KD/KO Validated

GNAQ Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

7

Kat-Nr. : 13927-1-AP

Synonyme

G ALPHA q, GAQ, GNAQ

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Galerie der Validierungsdaten

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  WB analysis using 13927-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13927-1-AP (GNAQ antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse lung using 13927-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 13927-1-AP (GNAQ antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse skin using 13927-1-AP

  mouse skin tissue were subjected to SDS PAGE followed by western blot with 13927-1-AP (GNAQ antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 13927-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 13927-1-AP (GNAQ antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human testis using 13927-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 13927-1-AP (GNAQ antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human testis using 13927-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 13927-1-AP (GNAQ antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human breast cancer using 13927-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13927-1-AP (GNAQ antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 13927-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13927-1-AP (GNAQ antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HEK-293 using 13927-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using GNAQ antibody (13927-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HeLa using 13927-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 13927-1-AP (GNAQ antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, humanes Hirngewebe, Jurkat-Zellen, Maushautgewebe, Maushirngewebe, Mauslungengewebe, NIH/3T3-Zellen, Rattenhirngewebe
• Erfolgreiche Detektion in IHC: humanes Hodengewebe, humanes Mammakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HEK-293-Zellen, HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 7 publications below
• IHC: See 1 publications below
• IF: See 3 publications below

Produktinformation

13927-1-AP bindet in WB, IHC, IF, ELISA GNAQ und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: GNAQ fusion protein Ag4947
• Vollständiger Name: guanine nucleotide binding protein (G protein), q polypeptide
• Berechnetes Molekulargewicht: 42 kDa
• Beobachtetes Molekulargewicht: 38-40 kDa
• GenBank-Zugangsnummer: BC057777
• Gene symbol: GNAQ
• Gene ID (NCBI): 2776
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

GNAQ is a proto-oncogene closely related to the α subunit of guanine nucleotide-binding proteins (G proteins) that serve as key intermediates between membrane-bound G-protein-coupled receptors (GPCRs) and GPCR signalling nodes. Aberrant expression and activity of G proteins and GPCRs are frequently associated with tumorigenesis. Recent studies show that oncogenic activating mutations in GNAQ are present in 80% of the melanomas. The calculated molecular weight of GNAQ is 42 kDa. This antibody can detect a band of 38-40 kDa in SDS-PAGE. (PMID: 33993733)

Publikationen

Species	Application	Title
mouse	WB	J Exp Med; Uncovering a novel role of PLCβ4 in selectively mediating TCR signaling in CD8+ but not CD4+ T cells.; Authors - Miwa Sasai
mouse	WB,IF	Elife; Gq activity- and β-arrestin-1 scaffolding-mediated ADGRG2/CFTR coupling are required for male fertility.; Authors - Dao-Lai Zhang
mouse	WB	Oncogene; Copper ionophore elesclomol selectively targets GNAQ/11-mutant uveal melanoma.; Authors - Yongyun Li
human	WB	Proteomics; iTRAQ-based proteomic analysis of dioscin on human HCT-116 colon cancer cells.; Authors - Hao Chen
human	WB,IHC	Oncol Rep; G protein subunit α q regulates gastric cancer growth via the p53/p21 and MEK/ERK pathways.; Authors - Yizhuo Wang ; KD Validated
human	WB,IF	J Biol Chem; Enhanced membrane binding of oncogenic G protein αqQ209L confers resistance to inhibitor YM-254890; Authors - Clinita E Randolph