GNAQ Monoklonaler Antikörper

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• KD/KO Validated

GNAQ Monoklonal Antikörper für WB, ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

Hausschwein, human, Kaninchen, Maus, Ratte

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

CloneNo.

2H5G6

Rezensionen (1)

Kat-Nr. : 68191-1-Ig

Synonyme

2H5G6, CMC1, G ALPHA q, GAQ, Guanine nucleotide-binding protein alpha-q

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  WB analysis using 68191-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68191-1-Ig (GNAQ antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 68191-1-Ig

  WB result of GNAQ antibody (68191-1-Ig; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GNAQ transfected MCF-7 cells.

• 

  WB analysis of pig cerebellum using 68191-1-Ig

  pig cerebellum tissue were subjected to SDS PAGE followed by western blot with 68191-1-Ig (GNAQ antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 68191-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68191-1-Ig (GNAQ antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat cerebellum using 68191-1-Ig

  rat cerebellum tissue were subjected to SDS PAGE followed by western blot with 68191-1-Ig (GNAQ antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 68191-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 68191-1-Ig (GNAQ antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Jurkat-Zellen, HeLa-Zellen, MCF-7-Zellen, Maushirngewebe, Hausschwein-Hirngewebe, Hausschwein-Cerebellum-Gewebe, Kaninchenhirngewebe, Rattenhirngewebe, Ratten-Cerebellum-Gewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

68191-1-Ig bindet in WB, ELISA GNAQ und zeigt Reaktivität mit Hausschwein, human, Kaninchen, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Kaninchen, Maus, Ratte
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: GNAQ fusion protein Ag26178
• Vollständiger Name: guanine nucleotide binding protein (G protein), q polypeptide
• Berechnetes Molekulargewicht: 42 kDa
• Beobachtetes Molekulargewicht: 38-40 kDa
• GenBank-Zugangsnummer: BC057777
• Gene symbol: GNAQ
• Gene ID (NCBI): 2776
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

GNAQ is a proto-oncogene closely related to the α subunit of guanine nucleotide-binding proteins (G proteins) that serve as key intermediates between membrane-bound G-protein-coupled receptors (GPCRs) and GPCR signalling nodes. Aberrant expression and activity of G proteins and GPCRs are frequently associated with tumorigenesis. Recent studies show that oncogenic activating mutations in GNAQ are present in 80% of the melanomas. The calculated molecular weight of GNAQ is 42 kDa. This antibody can detect a band of 38-40 kDa in SDS-PAGE. (PMID: 33993733)

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Udesh (Verified Customer) (12-29-2023)

Worked well for WB at 1:1000 in Glioma cells

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Glioblastoma