GNAS Monoklonaler Antikörper

GNAS Monoklonal Antikörper für IF/ICC, IF-P

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

human

Anwendung

IF/ICC, IF-P

Konjugation

CoraLite®594 Fluorescent Dye

CloneNo.

1A9C10

Kat-Nr. : CL594-66253

Synonyme

Neuroendocrine secretory protein 55, NESP55, LHAL tetrapeptide, GPIPIRRH peptide, GNAS1

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  IF Staining of human colon cancer using CL594-66253

  Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using CoraLite®594 GNAS antibody (CL594-66253, Clone: 1A9C10 ) at dilution of 1:200.

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  IF Staining of human colon cancer using CL594-66253

  Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using CoraLite®594 GNAS antibody (CL594-66253, Clone: 1A9C10 ) at dilution of 1:200.

• 

  IF Staining of human colon cancer using CL594-66253

  Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using CoraLite®594 GNAS antibody (CL594-66253, Clone: 1A9C10 ) at dilution of 1:200.

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  IF Staining of MCF-7 using CL594-66253

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using CoraLite®594 GNAS antibody (CL594-66253, Clone: 1A9C10 ) at dilution of 1:200.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF-P: humanes Kolonkarzinomgewebe
• Erfolgreiche Detektion in IF/ICC: MCF-7-Zellen

Empfohlene Verdünnung

• Immunfluoreszenz (IF)-P: IF-P : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

CL594-66253 bindet in IF/ICC, IF-P GNAS und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: GNAS fusion protein Ag20530
• Vollständiger Name: GNAS complex locus
• Berechnetes Molekulargewicht: 45 kDa
• Beobachtetes Molekulargewicht: 46 kDa
• GenBank-Zugangsnummer: BC002722
• Gene symbol: GNAS
• Gene ID (NCBI): 2778
• Konjugation: CoraLite®594 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 588 nm / 604 nm
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Guanine nucleotide binding protein (G protein), alpha stimulating activity polypeptide 1 (GNAS1) is the ubiquitously expressed heterotrimeric G protein that couples receptors to the effector enzyme adenylyl cyclase and is required for receptor-stimulated intracellular cAMP generation. Mutations of Gs(alpha) residues involved in the GTPase reaction that lead to constitutive activation are present in endocrine tumors, fibrous dysplasia of bone, and McCune-Albright syndrome. The molecular weight of Gs(alpha) protein is about 46 kDa.