GNB1 Polyklonaler Antikörper

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GNB1 Polyklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 10247-2-PBS

Synonyme

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  WB analysis using 10247-2-AP (same clone as 10247-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 10247-2-AP (GNB1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10247-2-PBS in a different storage buffer formulation.

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  IHC staining of human pancreas cancer using 10247-2-AP (same clone as 10247-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 10247-2-AP (GNB1 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 10247-2-PBS in a different storage buffer formulation.

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  IHC staining of human pancreas cancer using 10247-2-AP (same clone as 10247-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 10247-2-AP (GNB1 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 10247-2-PBS in a different storage buffer formulation.

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  IHC staining of human gliomas using 10247-2-AP (same clone as 10247-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human gliomas using 10247-2-AP (GNB1 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 10247-2-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 10247-2-AP (same clone as 10247-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using GNB1 antibody (10247-2-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 10247-2-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

10247-2-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA GNB1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: GNB1 fusion protein Ag0313
• Vollständiger Name: guanine nucleotide binding protein (G protein), beta polypeptide 1
• Berechnetes Molekulargewicht: 37 kDa
• Beobachtetes Molekulargewicht: 37 kDa
• GenBank-Zugangsnummer: BC004186
• Gene symbol: GNB1
• Gene ID (NCBI): 2782
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

GNB1(Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1) belongs to the WD repeat G protein beta family.The guanine nucleotide-binding proteins (G proteins) are involved as a modulator or transducer in various transmembrane signaling systems and the beta and gamma chains are required for the GTPase activity, for replacement of GDP by GTP, and for G protein-effector interaction.GNB1 can form dimers with all gamma subunits analyzed(PMID:12782285).

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