GNB2 Polyklonaler Antikörper

GNB2 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(2)

Kat-Nr. : 16090-1-AP

Synonyme

Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-2, G protein subunit beta-2

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  WB analysis of HeLa using 16090-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 16090-1-AP (GNB2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 16090-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 16090-1-AP (GNB2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 16090-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 16090-1-AP (GNB2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 16090-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 16090-1-AP (GNB2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human ovary tumor using 16090-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 16090-1-AP (GNB2 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human ovary tumor using 16090-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 16090-1-AP (GNB2 antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HeLa using 16090-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using GNB2 antibody (16090-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

• 

  IF Staining of HepG2 using 16090-1-AP

  Immunofluorescent analysis of HepG2 cells, using GNB2 antibody 16090-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HEK-293-Zellen, Jurkat-Zellen
• Erfolgreiche Detektion in IHC: humanes Ovarialkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen, HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below

Produktinformation

16090-1-AP bindet in WB, IHC, IF/ICC, ELISA GNB2 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: GNB2 fusion protein Ag9125
• Vollständiger Name: guanine nucleotide binding protein (G protein), beta polypeptide 2
• Berechnetes Molekulargewicht: 340 aa, 37 kDa
• Beobachtetes Molekulargewicht: 37 kDa
• GenBank-Zugangsnummer: BC010073
• Gene symbol: GNB2
• Gene ID (NCBI): 2783
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

GNB2(Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-2) is also named as G protein subunit beta-2, transducin beta chain 2 and belongs to the WD repeat G protein beta family. G proteins are involved as a modulator or transducer in various transmembrane signaling systems. The beta and gamma chains are required for the GTPase activity, for replacement of GDP by GTP, and for G protein-effector interaction.

Publikationen

Species	Application	Title
mouse		Front Cardiovasc Med; Macrophages-Related Genes Biomarkers in the Deterioration of Atherosclerosis.; Authors - Yue Zheng
human,mouse	WB	PLoS Biol; KCTD proteins regulate morphine dependence via heterologous sensitization of adenylyl cyclase 1 in mice; Authors - Zhong Ding