GNB2 Polyklonaler Antikörper

GNB2 Polyklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 16090-1-PBS

Synonyme

Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-2, G protein subunit beta-2

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  WB analysis of HeLa using 16090-1-AP (same clone as 16090-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 16090-1-AP (GNB2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16090-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 16090-1-AP (same clone as 16090-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 16090-1-AP (GNB2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16090-1-PBS in a different storage buffer formulation.

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  WB analysis of Jurkat using 16090-1-AP (same clone as 16090-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 16090-1-AP (GNB2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16090-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 16090-1-AP (same clone as 16090-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 16090-1-AP (GNB2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16090-1-PBS in a different storage buffer formulation.

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  IHC staining of human ovary tumor using 16090-1-AP (same clone as 16090-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 16090-1-AP (GNB2 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 16090-1-PBS in a different storage buffer formulation.

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  IHC staining of human ovary tumor using 16090-1-AP (same clone as 16090-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 16090-1-AP (GNB2 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 16090-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 16090-1-AP (same clone as 16090-1-PBS)

  Immunofluorescent analysis of HepG2 cells, using GNB2 antibody 16090-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). This data was developed using the same antibody clone with 16090-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 16090-1-AP (same clone as 16090-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using GNB2 antibody (16090-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 16090-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

16090-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA GNB2 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: GNB2 fusion protein Ag9125
• Vollständiger Name: guanine nucleotide binding protein (G protein), beta polypeptide 2
• Berechnetes Molekulargewicht: 340 aa, 37 kDa
• Beobachtetes Molekulargewicht: 37 kDa
• GenBank-Zugangsnummer: BC010073
• Gene symbol: GNB2
• Gene ID (NCBI): 2783
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

GNB2(Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-2) is also named as G protein subunit beta-2, transducin beta chain 2 and belongs to the WD repeat G protein beta family. G proteins are involved as a modulator or transducer in various transmembrane signaling systems. The beta and gamma chains are required for the GTPase activity, for replacement of GDP by GTP, and for G protein-effector interaction.