GNB3 Rekombinanter Antikörper

GNB3 Rekombinant Antikörper für ELISA, WB

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

ELISA, WB

Konjugation

Unkonjugiert

CloneNo.

240281D1

Kat-Nr. : 83452-4-PBS

Synonyme

Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-3, 240281D1

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  WB analysis using 83452-4-RR (same clone as 83452-4-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83452-4-RR (GNB3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83452-4-PBS in a different storage buffer formulation.

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  FC experiment of HepG2 using 83452-4-RR (same clone as 83452-4-PBS)

  1x10^6 HepG2 cells were intracellularly stained with 0.25 ug GNB3 Recombinant antibody (83452-4-RR, Clone:240281D1) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83452-4-PBS in a different storage buffer formulation.

• 

  FC experiment of HeLa using 83452-4-RR (same clone as 83452-4-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug GNB3 Recombinant antibody (83452-4-RR, Clone:240281D1) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83452-4-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 83452-4-RR

  Biolayer interferometry (BLl) kinetic assays of 83452-4-RR against Human GNB3 were performed. The affinity constant is below 1 pM.

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Geprüfte Anwendungen

Produktinformation

83452-4-PBS bindet in ELISA, WB GNB3 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: GNB3 fusion protein Ag0127
• Vollständiger Name: guanine nucleotide binding protein (G protein), beta polypeptide 3
• Berechnetes Molekulargewicht: 37 kDa
• GenBank-Zugangsnummer: BC000115
• Gene symbol: GNB3
• Gene ID (NCBI): 2784
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.