GNB4 Polyklonaler Antikörper

GNB4 Polyklonal Antikörper für WB, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(2)

Kat-Nr. : 11978-2-AP

Synonyme

HG2B, Guanine nucleotide-binding protein subunit beta-4, CMTD1F

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  WB analysis using 11978-2-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11978-2-AP (GNB4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 11978-2-AP

  IP result of anti-GNB4 (IP:11978-2-AP, 4ug; Detection:11978-2-AP 1:800) with mouse brain tissue lysate 4000ug.

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  IF Staining of HepG2 using 11978-2-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using GNB4 antibody (11978-2-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  IF Staining of SH-SY5Y using 11978-2-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using GNB4 antibody (11978-2-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A549-Zellen, HEK-293T-Zellen, humanes Plazenta-Gewebe, MCF-7-Zellen, Mausnierengewebe, SH-SY5Y-Zellen
• Erfolgreiche IP: Maushirngewebe
• Erfolgreiche Detektion in IF/ICC: SH-SY5Y-Zellen, HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below
• IF: See 1 publications below

Produktinformation

11978-2-AP bindet in WB, IF/ICC, IP, ELISA GNB4 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: GNB4 fusion protein Ag2604
• Vollständiger Name: guanine nucleotide binding protein (G protein), beta polypeptide 4
• Berechnetes Molekulargewicht: 340 aa, 38 kDa
• Beobachtetes Molekulargewicht: 38 kDa
• GenBank-Zugangsnummer: BC000873
• Gene symbol: GNB4
• Gene ID (NCBI): 59345
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

G protein subunit beta 4 (GNB4), is one of the heterotrimeric guanine nucleotide-binding proteins (G proteins), which integrate signals between receptors and effector proteins. GNB4 is important regulators of alpha subunits, as well as of certain signal transduction receptors and effectors.

Publikationen

Species	Application	Title
human	IF	BMC Med; Helicobacter pylori-induced aberrant demethylation and expression of GNB4 promotes gastric carcinogenesis via the Hippo-YAP1 pathway; Authors - Duanrui Liu
human	WB	Nat Commun; Cross-linking of the endolysosomal system reveals potential flotillin structures and cargo; Authors - Jasjot Singh