GNB5 Polyklonaler Antikörper

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• KD/KO Validated

GNB5 Polyklonal Antikörper für IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

5

Kat-Nr. : 11045-2-AP

Synonyme

GB5, Gbeta5, GNB5, Transducin beta chain 5

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Galerie der Validierungsdaten

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  WB analysis of mouse brain using 11045-2-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 11045-2-AP (GNB5 Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 11045-2-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 11045-2-AP (GNB5 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IP experiment of rat brain using 11045-2-AP

  IP Result of anti-GNB5 (IP:11045-2-AP, 4ug; Detection:11045-2-AP 1:500) with rat brain tissue lysate 4000ug.

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  IHC staining of human ovary tumor using 11045-2-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 11045-2-AP (GNB5 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary tumor using 11045-2-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 11045-2-AP (GNB5 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary tumor using 11045-2-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 11045-2-AP (GNB5 antibody) at dilution of 1:100 (under 10x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, humanes Hirngewebe
• Erfolgreiche IP: Rattenhirngewebe
• Erfolgreiche Detektion in IHC: humanes Ovarialkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 5 publications below
• IF: See 1 publications below

Produktinformation

11045-2-AP bindet in WB, IP, IHC, IF, ELISA GNB5 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: GNB5 fusion protein Ag1518
• Vollständiger Name: guanine nucleotide binding protein (G protein), beta 5
• Berechnetes Molekulargewicht: 39 kDa
• Beobachtetes Molekulargewicht: 39-42 kDa
• GenBank-Zugangsnummer: BC011671
• Gene symbol: GNB5
• Gene ID (NCBI): 10681
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Guanine nucleotide-binding proteins (G proteins) are involved as a modulator or transducer in various transmembrane signaling systems. The beta and gamma chains are required for the GTPase activity, for replacement of GDP by GTP, and for G protein-effector interaction. G proteins, which integrate signals between receptors and effector proteins, are composed of an alpha, a beta, and a gamma subunit.

Publikationen

Species	Application	Title
human	WB	Oncogene; The Gβ5 protein regulates sensitivity to TRAIL-induced cell death in colon carcinoma.; Authors - D Fuchs ; KD Validated
rat	WB	J Cell Mol Med; Prenatal hypoxia inhibited propionate-evoked BK channels of mesenteric artery smooth muscle cells in offspring.; Authors - Wenna Zhang
mouse	WB	Neuropharmacology.; A systems genetic analysis of alcohol drinking by mice, rats and men: Influence of brain GABAergic transmission.; Authors - Saba LM
mouse	WB, IF	PMC Biophys; Retinal cone photoreceptors require phosducin-like protein 1 for g protein complex assembly and signaling.; Authors - Christopher M Tracy
human	WB	FEBS J; PMID: 30719834; Authors - Sang-Min Park ; KD Validated