GNL3 Polyklonaler Antikörper

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• KD/KO Validated

GNL3 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(2)

Kat-Nr. : 15060-1-AP

Synonyme

NNP47, Guanine nucleotide-binding protein-like 3, E2-induced gene 3 protein, E2IG3, E2 induced gene 3 protein

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  WB analysis using 15060-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15060-1-AP (Nucleostemin antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 15060-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 15060-1-AP (Nucleostemin antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 15060-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15060-1-AP (Nucleostemin antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 15060-1-AP

  IP result of anti-GNL3 (IP:15060-1-AP, 4ug; Detection:15060-1-AP 1:600) with HeLa cells lysate 4000ug.

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  IHC staining of human oesophagus cancer using 15060-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 15060-1-AP (GNL3 antibody) at dilution of 1:1600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human oesophagus cancer using 15060-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 15060-1-AP (GNL3 antibody) at dilution of 1:1600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 15060-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15060-1-AP (GNL3 antibody) at dilution of 1:1600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 15060-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15060-1-AP (GNL3 antibody) at dilution of 1:1600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 15060-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 15060-1-AP (GNL3 antibody), at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, HeLa-Zellen, HepG2-Zellen, K-562-Zellen, Raji-Zellen
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Ösophaguskarzinomgewebe, humanes Kolonkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:800-1:3200
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 2 publications below

Produktinformation

15060-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA GNL3 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: GNL3 fusion protein Ag7056
• Vollständiger Name: guanine nucleotide binding protein-like 3 (nucleolar)
• Berechnetes Molekulargewicht: 62 kDa
• Beobachtetes Molekulargewicht: 62 kDa
• GenBank-Zugangsnummer: BC001024
• Gene symbol: GNL3
• Gene ID (NCBI): 26354
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB	J Nanobiotechnology; Targeted micelles with chemotherapeutics and gene drugs to inhibit the G1/S and G2/M mitotic cycle of prostate cancer.; Authors - Yiran Zhang
human	WB	Cancer Manag Res; The oncogenic role of GNL3 in the progression and metastasis of osteosarcoma.; Authors - Tianyou Li ; KD Validated