GNMT Monoklonaler Antikörper

GNMT Monoklonal Antikörper für WB, IF/ICC, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human, Maus, Ratte

Anwendung

WB, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

1B5E3

Kat-Nr. : 67294-1-PBS

Synonyme

EC:2.1.1.20, glycine N methyltransferase, Glycine N-methyltransferase

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  WB analysis using 67294-1-Ig (same clone as 67294-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67294-1-Ig (GNMT antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67294-1-PBS in a different storage buffer formulation.

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  WB analysis of pig liver using 67294-1-Ig (same clone as 67294-1-PBS)

  pig liver tissue were subjected to SDS PAGE followed by western blot with 67294-1-Ig (GNMT antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67294-1-PBS in a different storage buffer formulation.

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  WB analysis of rat liver using 67294-1-Ig (same clone as 67294-1-PBS)

  rat liver tissue were subjected to SDS PAGE followed by western blot with 67294-1-Ig (GNMT antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67294-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse liver using 67294-1-Ig (same clone as 67294-1-PBS)

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 67294-1-Ig (GNMT antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67294-1-PBS in a different storage buffer formulation.

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  IF Staining of A431 using 67294-1-Ig (same clone as 67294-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using GNMT antibody (67294-1-Ig, Clone: 1B5E3 ) at dilution of 1:800 and CoraLite®594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67294-1-PBS in a different storage buffer formulation.

• 

  IF Staining of A431 using 67294-1-Ig (same clone as 67294-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using GNMT antibody (67294-1-Ig, Clone: 1B5E3 ) at dilution of 1:800 and CoraLite®594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67294-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

67294-1-PBS bindet in WB, IF/ICC, Indirect ELISA GNMT und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: GNMT fusion protein Ag4598
• Vollständiger Name: glycine N-methyltransferase
• Berechnetes Molekulargewicht: 295 aa, 33 kDa
• Beobachtetes Molekulargewicht: 33 kDa
• GenBank-Zugangsnummer: BC032627
• Gene symbol: GNMT
• Gene ID (NCBI): 27232
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Glycine N-methyltransferase (GNMT, EC 2.1.1.20) was found originally as an enzyme regulating the ratio of SAM to S-adenosyl- homocysteine. GNMT is conservative among different animal species. Glycine-N methyltransferase (GNMT) is a potential tumor suppressor that is commonly inactivated in human hepatoma. GNMT is abundant in liver, but very low in HepG2 cells (PMID: 12566990).