GNPDA1 Polyklonaler Antikörper

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• KD/KO Validated

GNPDA1 Polyklonal Antikörper für IHC, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

3

Kat-Nr. : 12312-1-AP

Synonyme

GlcN6P deaminase 1, GNPDA, GNPDA 1, GNPDA1, GNPI, GPI, HLN, KIAA0060, Oscillin

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Galerie der Validierungsdaten

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  WB analysis of K-562 using 12312-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 12312-1-AP (GNPDA1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 12312-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 12312-1-AP (GNPDA1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 12312-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 12312-1-AP (GNPDA1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of rat kidney using 12312-1-AP

  rat kidney tissue were subjected to SDS PAGE followed by western blot with 12312-1-AP (GNPDA1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human pancreas cancer using 12312-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 12312-1-AP (GNPDA1 antibody) at dilution of 1:50 (under 10x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: K-562-Zellen, HeLa-Zellen, humanes Hirngewebe, Rattennierengewebe
• Erfolgreiche Detektion in IHC: humanes Pankreaskarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 2 publications below
• IHC: See 1 publications below

Produktinformation

12312-1-AP bindet in WB, IHC, ELISA GNPDA1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: GNPDA1 fusion protein Ag2961
• Vollständiger Name: glucosamine-6-phosphate deaminase 1
• Berechnetes Molekulargewicht: 289 aa, 33 kDa
• Beobachtetes Molekulargewicht: 33 kDa
• GenBank-Zugangsnummer: BC012853
• Gene symbol: GNPDA1
• Gene ID (NCBI): 10007
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

GNPDA1(glucosamine-6-phosphate isomerase 1) is also named as GNPI, HLN, KIAA0060 and belongs to the glucosamine/galactosamine-6-phosphate isomerase family. It catalyzes the reversible conversion of D-glucosamine-6-phosphate (GlcN6P) into D-fructose-6-phosphate (Fru6P) and ammonium(PMID:12965206). It may be playing an essential role for egg activation and early embryo development. GNPI is expressed as a 33-kD cytosolic protein in various cell lines(PMID: 9714720).

Publikationen

Species	Application	Title
human	IHC	Front Cell Dev Biol; Identification and Validation of a Nine-Gene Amino Acid Metabolism-Related Risk Signature in HCC.; Authors - Yajuan Zhao
human	WB	Cancer Manag Res; Glucosamine-6-Phosphate Isomerase 1 Promotes Tumor Progression and Indicates Poor Prognosis in Hepatocellular Carcinoma.; Authors - Dezhi Li ; KD Validated
human	WB	Glycobiology; Hexosamine biosynthesis in keratinocytes: roles of GFAT and GNPDA enzymes in the maintenance of UDP-GlcNAc content and hyaluronan synthesis.; Authors - Sanna Oikari ; KD Validated