GNRHR Polyklonaler Antikörper

GNRHR Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen(1)

Kat-Nr. : 22462-1-AP

Synonyme

GnRH R, GnRH receptor, GNRHR, GNRHR1, GRHR, LHRHR, LRHR

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  WB analysis of mouse ovary using 22462-1-AP

  mouse ovary tissue were subjected to SDS PAGE followed by western blot with 22462-1-AP (GNRHR Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse testis using 22462-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 22462-1-AP (GNRHR Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human testis using 22462-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 22462-1-AP (GNRHR Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human testis using 22462-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 22462-1-AP (GNRHR Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of rat testis using 22462-1-AP

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 22462-1-AP (GNRHR antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of rat testis using 22462-1-AP

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 22462-1-AP (GNRHR antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of mouse testis using 22462-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 22462-1-AP (GNRHR antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 22462-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 22462-1-AP (GNRHR antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human testis using 22462-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 22462-1-AP (GNRHR Antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human testis using 22462-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 22462-1-AP (GNRHR Antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human pituitary adenoma using 22462-1-AP

  Immunohistochemical analysis of paraffin-embedded human pituitary adenoma tissue slide using 22462-1-AP (GNRHR Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pituitary adenoma using 22462-1-AP

  Immunohistochemical analysis of paraffin-embedded human pituitary adenoma tissue slide using 22462-1-AP (GNRHR Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maus-Eierstockgewebe, Maushodengewebe
• Erfolgreiche Detektion in IHC: humanes Hodengewebe, humanes Hypophysenadenom-Gewebe, Maushodengewebe, Rattenhodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IHC: See 1 publications below

Produktinformation

22462-1-AP bindet in WB, IHC, ELISA GNRHR und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: GNRHR fusion protein Ag18005
• Vollständiger Name: GnRH receptor
• Berechnetes Molekulargewicht: 328 aa, 38 kDa
• Beobachtetes Molekulargewicht: 60 kDa, 50 kDa
• GenBank-Zugangsnummer: BC113546
• Gene symbol: GNRHR
• Gene ID (NCBI): 2798
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

GNRHR, also named GRHR, belongs to the G-protein coupled receptor 1 family. GNRHR is a receptor for GnRH that mediates the action of GnRH to stimulate the secretion of the gonadotropic hormones luteinizing hormone (LH) and follicle-stimulating hormone (FSH). It mediates its action by association with G-proteins that activate a phosphatidylinositol-calcium second messenger system. Isoform2 of GNRHR may act as an inhibitor of GnRH-R signaling. Defects in GNRHR are a cause of idiopathic hypogonadotropic hypogonadism (IHH). Defects in GNRHR are a cause of fertile eunuch syndrome. The antibody only recognizes the isoform1 of GNRHR. The predicted unmodified molecular weight of the human GNRHR is ~38 kDa, the larger band (50-65 kDa) is likely to represent a glycosylated form of GNRHR.

Publikationen

Species	Application	Title
rat	IHC	Food Chem Toxicol; Prenatal genistein exposure in rats affects pubertal onset and serum reproductive hormone levels in male offsprings via the hypothalamic-pituitary-gonadal axis; Authors - Jing Zi