GOLGA2/GM130 Polyklonaler Antikörper

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• KD/KO Validated

GOLGA2/GM130 Polyklonal Antikörper für WB, IHC, IF/ICC, FC (Intra), ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Hund

Anwendung

WB, IHC, IF/ICC, FC (Intra), ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 11308-1-PBS

Synonyme

GM130, GOLGA2, 130 kDa cis-Golgi matrix protein, GM130 autoantigen, GM130;GOLGA2

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  WB analysis using 11308-1-AP (same clone as 11308-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 11308-1-AP (GOLGA2/GM130 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11308-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 11308-1-AP (same clone as 11308-1-PBS)

  WB result of GOLGA2/GM130 antibody (11308-1-AP; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GOLGA2/GM130 transfected HeLa cells. This data was developed using the same antibody clone with 11308-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 11308-1-AP (same clone as 11308-1-PBS)

  WB result of GOLGA2/GM130 antibody (11308-1-AP; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GOLGA2/GM130 transfected HEK-293 cells. This data was developed using the same antibody clone with 11308-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 11308-1-AP (same clone as 11308-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 11308-1-AP (GOLGA2/GM130 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11308-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 11308-1-AP (same clone as 11308-1-PBS)

  HEK-293 cells and HEK-293-derived exosomes (HEK-293-exo) were subjected to SDS PAGE followed by western blot with 11308-1-AP (GOLGA2/GM130 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11308-1-PBS in a different storage buffer formulation.

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  WB analysis of human spleen using 11308-1-AP (same clone as 11308-1-PBS)

  human spleen tissue were subjected to SDS PAGE followed by western blot with 11308-1-AP (GOLGA2,GM130 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11308-1-PBS in a different storage buffer formulation.

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  IHC staining of human testis using 11308-1-AP (same clone as 11308-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis using 11308-1-AP (GOLGA2,GM130 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 11308-1-PBS in a different storage buffer formulation.

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  IHC staining of human testis using 11308-1-AP (same clone as 11308-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis using 11308-1-AP (GOLGA2,GM130 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 11308-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 11308-1-AP (same clone as 11308-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using GOLGA2/GM130 antibody (11308-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red). This data was developed using the same antibody clone with 11308-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HEK-293 using 11308-1-AP (same clone as 11308-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using GOLGA2/GM130 antibody (11308-1-AP) at dilution of 1:4000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 11308-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 11308-1-AP (same clone as 11308-1-PBS)

  Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using 11308-1-AP (GOLGA2/GM130 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 11308-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 11308-1-AP (same clone as 11308-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using GOLGA2/GM130 antibody (11308-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red). This data was developed using the same antibody clone with 11308-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 11308-1-AP (same clone as 11308-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using GOLGA2/GM130 antibody (11308-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 11308-1-PBS in a different storage buffer formulation.

• 

  IF Staining of MDCK using 11308-1-AP (same clone as 11308-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed MDCK cells using 11308-1-AP (GOLGA2/GM130 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 11308-1-PBS in a different storage buffer formulation.

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  FC experiment of HepG2 using 11308-1-AP (same clone as 11308-1-PBS)

  1x10^6 HepG2 cells were intracellularly stained with 0.2 ug GOLGA2/GM130 Polyclonal antibody (11308-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.2 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 11308-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

11308-1-PBS bindet in WB, IHC, IF/ICC, FC (Intra), ELISA GOLGA2/GM130 und zeigt Reaktivität mit human, Hund

• Getestete Reaktivität: human, Hund
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: GOLGA2/GM130 fusion protein Ag1848
• Vollständiger Name: golgi autoantigen, golgin subfamily a, 2
• Berechnetes Molekulargewicht: 111 kDa
• Beobachtetes Molekulargewicht: 130 kDa
• GenBank-Zugangsnummer: BC014188
• Gene symbol: GM130
• Gene ID (NCBI): 2801
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

GOLGA2, also known as GM130, is a 130 kDa cis-Golgi matrix protein which is one component of the detergent and salt resistant Golgi matrix. It is a peripheral membrane protein highly bound to Golgi membrane and localized mainly at the cytoplasmic face of cis-Golgi membrane. Together with its interacting partner proteins, including p115, giantin, GRASP65, and Rab GTPase, GOLGA2/GM130 is involved in the regulation of ER-to-Golgi transport and also in the maintenance of the Golgi structure. Emerging evidence suggest that the GOLGA2/GM130 has potential roles in the control of glycosylation, cell cycle progression, and higher order cell functions such as cell polarization and directed cell migration. (PMID: 20197635)