GOLGA2/GM130 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

GOLGA2/GM130 Polyklonal Antikörper für IF/ICC

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

IF/ICC

Konjugation

CoraLite® Plus 488 Fluorescent Dye

Publikationen(1)

Kat-Nr. : CL488-11308

Synonyme

Cell and organelle markers, GM130, GM130 autoantigen, GOLGA2, GOLGA2/GM130, Golgi Marker, Golgin 95, Golgin subfamily A member 2

---

• 

  IF Staining of HeLa using CL488-11308

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 488 GOLGA2/GM130 antibody (CL488-11308) at dilution of 1:200, CoraLite®594 Coilin antibody (CL594-10967, red).

• 

  IF Staining of HepG2 using CL488-11308

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite® Plus 488 GOLGA2/GM130 antibody (CL488-11308) at dilution of 1:200.

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen, HepG2-Zellen

Empfohlene Verdünnung

• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IF: See 1 publications below

Produktinformation

CL488-11308 bindet in IF/ICC GOLGA2/GM130 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: GOLGA2/GM130 fusion protein Ag1848
• Vollständiger Name: golgi autoantigen, golgin subfamily a, 2
• Berechnetes Molekulargewicht: 111 kDa
• GenBank-Zugangsnummer: BC014188
• Gene symbol: GM130
• Gene ID (NCBI): 2801
• Konjugation: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

GOLGA2, also known as GM130, is a 130 kDa cis-Golgi matrix protein which is one component of the detergent and salt resistant Golgi matrix. It is a peripheral membrane protein highly bound to Golgi membrane and localized mainly at the cytoplasmic face of cis-Golgi membrane. Together with its interacting partner proteins, including p115, giantin, GRASP65, and Rab GTPase, GOLGA2/GM130 is involved in the regulation of ER-to-Golgi transport and also in the maintenance of the Golgi structure. Emerging evidence suggests that the GOLGA2/GM130 has potential roles in the control of glycosylation, cell cycle progression, and higher order cell functions such as cell polarization and directed cell migration. (PMID: 20197635)

Publikationen

Species	Application	Title
mouse	IF	Elife; TMC7 deficiency causes acrosome biogenesis defects and male infertility in mice; Authors - Jing Wang