GOLIM4 Rekombinanter Antikörper

GOLIM4 Rekombinant Antikörper für WB, IF/ICC, FC (Intra), ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IF/ICC, FC (Intra), ELISA

Konjugation

Unkonjugiert

CloneNo.

242323F4

Kat-Nr. : 84818-5-RR

Synonyme

242323F4, GIMPC, GOLPH4, GPP130, P 138

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  WB analysis using 84818-5-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84818-5-RR (GOLIM4 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 84818-5-RR

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using GOLIM4 antibody (84818-5-RR, Clone: 242323F4 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CoraLite®594-Phalloidin (red).

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  FC experiment of U-2 OS using 84818-5-RR

  1x10^6 U-2 OS cells were intracellularly stained with 0.25 ug GOLIM4 Recombinant antibody (84818-5-RR, Clone:242323F4) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

• 

  FC experiment of HeLa using 84818-5-RR

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug GOLIM4 Recombinant antibody (84818-5-RR, Clone:242323F4) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  Affinity and Kinetic Characterization of 84818-5-RR

  Biolayer interferometry (BLl) kinetic assays of 84818-5-RR against Human GOLIM4 were performed. The affinity constant is 0.926 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, C2C12-Zellen, HEK-293-Zellen, HepG2-Zellen, HT-1080.Zellen, L02-Zellen, MCF-7-Zellen
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen
• Erfolgreiche Detektion in FC (Intra): U-2 OS, HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:500-1:2000
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

84818-5-RR bindet in WB, IF/ICC, FC (Intra), ELISA GOLIM4 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: GOLIM4 fusion protein Ag34962
• Vollständiger Name: golgi integral membrane protein 4
• Berechnetes Molekulargewicht: 82 kDa
• Beobachtetes Molekulargewicht: 130-140 kDa
• GenBank-Zugangsnummer: NM_014498
• Gene symbol: GOLIM4
• Gene ID (NCBI): 27333
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Golgi integral membrane protein 4 (GOLIM4) is a 130-kDa membrane protein that is integrated into the cis/medial Golgi apparatus, which is a kind II Golgi protein. GOLIM4 is a Golgi protein that circulates between Golgi apparatus and endosomes, and its shuttle is pH sensitive(PMID:30068697).