GORAB Polyklonaler Antikörper

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• KD/KO Validated

GORAB Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IF, ELISA

Konjugation

Unkonjugiert

Publikationen(5)

Kat-Nr. : 17798-1-AP

Synonyme

NTKL binding protein 1, N-terminal kinase-like-binding protein 1, hNTKL-BP1, hNTKL BP1, golgin, RAB6 interacting

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  WB analysis using 17798-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17798-1-AP (GORAB antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse lung using 17798-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 17798-1-AP (GORAB antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, HeLa-Zellen, K-562-Zellen, Mauslungengewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:5000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 3 publications below
• IF: See 3 publications below

Produktinformation

17798-1-AP bindet in WB, IF, ELISA GORAB und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: GORAB fusion protein Ag12002
• Vollständiger Name: golgin, RAB6-interacting
• Berechnetes Molekulargewicht: 264aa,28 kDa; 394aa,45 kDa
• Beobachtetes Molekulargewicht: 45-50 kDa
• GenBank-Zugangsnummer: BC064945
• Gene symbol: GORAB
• Gene ID (NCBI): 92344
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

GORAB, also known as Scyl1BP1, is a protein that localizes to the trans-side of the Golgi apparatus and plays a significant role in intracellular trafficking and protein glycosylation. It is composed of a central coiled-coil region that is responsible for Golgi targeting, likely through interactions with small GTPases Rab6 and Arf5. GORAB is considered a member of the golgin family of coiled-coil Golgi proteins, which are involved in vesicle tethering. GORAB has been implicated in several cellular functions. It has been proposed to function as a transcriptional activator for neurite outgrowth, as a modulator of MDM2 ubiquitylation impacting p53 levels and apoptosis, and has been shown to play a role in centriole duplication and ciliogenesis.

Publikationen

Species	Application	Title
mouse	WB,IF	J Invest Dermatol; Gorab Is Required for Dermal Condensate Cells to Respond to Hedgehog Signals during Hair Follicle Morphogenesis.; Authors - Ying Liu ; KO Validated
mouse	WB,IF	FASEB J; GORAB promotes embryonic lung maturation through antagonizing AKT phosphorylation, versican expression, and mesenchymal cell migration.; Authors - Ying Liu ; KO Validated
human	IF	Methods Mol Biol; Immunofluorescence Microscopy of the Mammalian Golgi Apparatus; Authors - Maryam Arab
		J Cell Sci; Vimentin intermediate filaments provide structural stability to the mammalian Golgi complex; Authors - Teresa Vitali
human	WB	Mol Vis; Identification and functional analysis of a novel oculocerebrorenal syndrome of Lowe (OCRL) gene variant in two pedigrees with varying phenotypes including isolated congenital cataract.; Authors - Ahmed K Shalaby