GORASP2 Monoklonaler Antikörper

GORASP2 Monoklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

4

CloneNo.

1C9A3

Rezensionen (1)

Kat-Nr. : 66627-1-Ig

Synonyme

Golgi phosphoprotein 6, GOLPH6, GORASP2, GRASP55, GRS2, p59

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Galerie der Validierungsdaten

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  WB analysis using 66627-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66627-1-Ig (GORASP2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis of pig brain using 66627-1-Ig

  pig brain tissue were subjected to SDS PAGE followed by western blot with 66627-1-Ig (GORASP2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat brain using 66627-1-Ig

  rat brain tissue were subjected to SDS PAGE followed by western blot with 66627-1-Ig (GORASP2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 66627-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66627-1-Ig (GORASP2 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 66627-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66627-1-Ig (GORASP2 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 66627-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using GORASP2 antibody (66627-1-Ig, Clone: 1C9A3 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A549-Zellen, Hausschwein-Hirngewebe, HEK-293-Zellen, HeLa-Zellen, HepG2-Zellen, HL-60-Zellen, Jurkat-Zellen, K-562-Zellen, LNCaP-Zellen, NCCIT-Zellen, Rattenhirngewebe
• Erfolgreiche Detektion in IHC: humanes Kolonkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:250-1:1000
• Immunfluoreszenz (IF): IF : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below
• IF: See 4 publications below

Produktinformation

66627-1-Ig bindet in WB, IHC, IF, ELISA GORASP2 und zeigt Reaktivität mit Hausschwein, human, Ratten

• Getestete Reaktivität: Hausschwein, human, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: GORASP2 fusion protein Ag17607
• Vollständiger Name: golgi reassembly stacking protein 2, 55kDa
• Berechnetes Molekulargewicht: 55 kDa
• Beobachtetes Molekulargewicht: 59 kDa
• GenBank-Zugangsnummer: BC007770
• Gene symbol: GORASP2
• Gene ID (NCBI): 26003
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

GORASP2 (Golgi reassembly-stacking protein 2, or GRS2) has multiple phosphorylation sites and can be phosphorylated by MKK/ERK pathway during mitoisis. GORASP2 plays a role in the assembly and membrane stacking of the Golgi cisternae, and in the process by which Golgi stacks reform after mitotic breakdown.GORASP2 is supposed to regulate the intracellular transport and presentation of a defined set of transmembrane proteins, such as transmembrane TGFA.

Publikationen

Species	Application	Title
human	WB,IF	Mol Cell; An mTORC1-GRASP55 signaling axis controls unconventional secretion to reshape the extracellular proteome upon stress.; Authors - Julian Nüchel
human	WB,IF	J Cell Biol; Rapid degradation of GRASP55 and GRASP65 reveals their immediate impact on the Golgi structure.; Authors - Yijun Zhang
human	IF	Front Cell Dev Biol; Long-term culture of patient-derived cardiac organoids recapitulated Duchenne muscular dystrophy cardiomyopathy and disease progression; Authors - Vittoria Marini
	IF	Cell Death Dis; A cross-talk between epithelium and endothelium mediates human alveolar-capillary injury during SARS-CoV-2 infection.; Authors - Peng Wang

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Boyan (Verified Customer) (01-11-2020)

This antibody can be used for detecting the Golgi localisation of endogenous Gorasp2.

• Applications: Immunofluorescence,