GORASP2 Monoklonaler Antikörper

GORASP2 Monoklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human, Ratte

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(6)

CloneNo.

1C9A3

Kat-Nr. : 66627-1-Ig

Synonyme

1C9A3, Golgi phosphoprotein 6, Golgi reassembly-stacking protein 2, Golgi reassembly-stacking protein of 55 kDa, GOLPH6



Geprüfte Anwendungen

Erfolgreiche Detektion in WBA549-Zellen, HEK-293-Zellen, HeLa-Zellen, HepG2-Zellen, HL-60-Zellen, Jurkat-Zellen, K-562-Zellen, LNCaP-Zellen, NCCIT-Zellen, Hausschwein-Hirngewebe, Rattenhirngewebe
Erfolgreiche Detektion in IHChumanes Kolonkarzinomgewebe
Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
Erfolgreiche Detektion in IF/ICCHepG2-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:5000-1:50000
Immunhistochemie (IHC)IHC : 1:250-1:1000
Immunfluoreszenz (IF)/ICCIF/ICC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

WBSee 3 publications below
IFSee 5 publications below

Produktinformation

66627-1-Ig bindet in WB, IHC, IF/ICC, ELISA GORASP2 und zeigt Reaktivität mit Hausschwein, human, Ratten

Getestete Reaktivität Hausschwein, human, Ratte
In Publikationen genannte Reaktivitäthuman, Ratte
Wirt / Isotyp Maus / IgG1
Klonalität Monoklonal
Typ Antikörper
Immunogen GORASP2 fusion protein Ag17607
Vollständiger Name golgi reassembly stacking protein 2, 55kDa
Berechnetes Molekulargewicht 55 kDa
Beobachtetes Molekulargewicht 59 kDa
GenBank-ZugangsnummerBC007770
Gene symbol GORASP2
Gene ID (NCBI) 26003
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer PBS with 0.02% sodium azide and 50% glycerol
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

GORASP2 (Golgi reassembly-stacking protein 2, or GRS2) has multiple phosphorylation sites and can be phosphorylated by MKK/ERK pathway during mitoisis. GORASP2 plays a role in the assembly and membrane stacking of the Golgi cisternae, and in the process by which Golgi stacks reform after mitotic breakdown.GORASP2 is supposed to regulate the intracellular transport and presentation of a defined set of transmembrane proteins, such as transmembrane TGFA.

Protokolle

PRODUKTSPEZIFISCHE PROTOKOLLE
WB protocol for GORASP2 antibody 66627-1-IgProtokoll herunterladen
IHC protocol for GORASP2 antibody 66627-1-IgProtokoll herunterladenl
IF protocol for GORASP2 antibody 66627-1-IgProtokoll herunterladen
STANDARD-PROTOKOLLE
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
humanWB,IF

Mol Cell

An mTORC1-GRASP55 signaling axis controls unconventional secretion to reshape the extracellular proteome upon stress.

Authors - Julian Nüchel
humanWB,IF

J Cell Biol

Rapid degradation of GRASP55 and GRASP65 reveals their immediate impact on the Golgi structure.

Authors - Yijun Zhang
humanIF

Front Cell Dev Biol

Long-term culture of patient-derived cardiac organoids recapitulated Duchenne muscular dystrophy cardiomyopathy and disease progression

Authors - Vittoria Marini
IF

Cell Death Dis

A cross-talk between epithelium and endothelium mediates human alveolar-capillary injury during SARS-CoV-2 infection.

Authors - Peng Wang
humanWB

Cell Commun Signal

CHOP upregulation and dysregulation of the mature form of the SNAT2 amino acid transporter in the placentas from small for gestational age newborns

Authors - Emma Barroso
ratIF

Cell Signal

Targeting CDK1 inhibits Golgi apparatus stress-mediated neuroinflammation and neuronal apoptosis after intracerebral hemorrhage by modulating GRASP55 phosphorylation

Authors - Xiaobo Zhang

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

Boyan (Verified Customer) (01-11-2020)

This antibody can be used for detecting the Golgi localisation of endogenous Gorasp2.

  • Applications: Immunofluorescence,