GORASP2 Monoklonaler Antikörper

GORASP2 Monoklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human, Ratte

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

1C9A3

Kat-Nr. : 66627-1-PBS

Synonyme

1C9A3, Golgi phosphoprotein 6, Golgi reassembly-stacking protein 2, Golgi reassembly-stacking protein of 55 kDa, GOLPH6

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  WB analysis using 66627-1-Ig (same clone as 66627-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66627-1-Ig (GORASP2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66627-1-PBS in a different storage buffer formulation.

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  WB analysis of pig brain using 66627-1-Ig (same clone as 66627-1-PBS)

  pig brain tissue were subjected to SDS PAGE followed by western blot with 66627-1-Ig (GORASP2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66627-1-PBS in a different storage buffer formulation.

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  WB analysis of rat brain using 66627-1-Ig (same clone as 66627-1-PBS)

  rat brain tissue were subjected to SDS PAGE followed by western blot with 66627-1-Ig (GORASP2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66627-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 66627-1-Ig (same clone as 66627-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66627-1-Ig (GORASP2 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66627-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 66627-1-Ig (same clone as 66627-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66627-1-Ig (GORASP2 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66627-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 66627-1-Ig (same clone as 66627-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using GORASP2 antibody (66627-1-Ig, Clone: 1C9A3 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66627-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

66627-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA GORASP2 und zeigt Reaktivität mit Hausschwein, human, Ratten

• Getestete Reaktivität: Hausschwein, human, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: GORASP2 fusion protein Ag17607
• Vollständiger Name: golgi reassembly stacking protein 2, 55kDa
• Berechnetes Molekulargewicht: 55 kDa
• Beobachtetes Molekulargewicht: 59 kDa
• GenBank-Zugangsnummer: BC007770
• Gene symbol: GORASP2
• Gene ID (NCBI): 26003
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

GORASP2 (Golgi reassembly-stacking protein 2, or GRS2) has multiple phosphorylation sites and can be phosphorylated by MKK/ERK pathway during mitoisis. GORASP2 plays a role in the assembly and membrane stacking of the Golgi cisternae, and in the process by which Golgi stacks reform after mitotic breakdown.GORASP2 is supposed to regulate the intracellular transport and presentation of a defined set of transmembrane proteins, such as transmembrane TGFA.