GORASP2 Rekombinanter Antikörper

GORASP2 Rekombinant Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

242700C11

Kat-Nr. : 85077-5-RR

Synonyme

242700C11, Golgi phosphoprotein 6, Golgi reassembly-stacking protein 2, Golgi reassembly-stacking protein of 55 kDa, GOLPH6

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  WB analysis using 85077-5-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85077-5-RR (GORASP2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 85077-5-RR

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 85077-5-RR (GORASP2 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 85077-5-RR

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 85077-5-RR (GORASP2 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 85077-5-RR

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using GORASP2 antibody (85077-5-RR, Clone: 242700C11 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  IF Staining of HepG2 using 85077-5-RR

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using GORASP2 antibody (85077-5-RR, Clone: 242700C11 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  IF Staining of U2OS using 85077-5-RR

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using GORASP2 antibody (85077-5-RR, Clone: 242700C11 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

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  Affinity and Kinetic Characterization of 85077-5-RR

  Biolayer interferometry (BLl) kinetic assays of 85077-5-RR against Human GORASP2 were performed. The affinity constant is 5.33 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HCT 116-Zellen, COLO 320-Zellen, HeLa-Zellen, Raji-Zellen
• Erfolgreiche Detektion in IHC: humanes Kolonkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen, U2OS-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:250-1:1000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

85077-5-RR bindet in WB, IHC, IF/ICC, ELISA GORASP2 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: GORASP2 fusion protein Ag0950
• Vollständiger Name: golgi reassembly stacking protein 2, 55kDa
• Berechnetes Molekulargewicht: 55 kDa
• Beobachtetes Molekulargewicht: 59 kDa
• GenBank-Zugangsnummer: BC007770
• Gene symbol: GORASP2
• Gene ID (NCBI): 26003
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

GORASP2 (Golgi reassembly-stacking protein 2, or GRS2) has multiple phosphorylation sites and can be phosphorylated by MKK/ERK pathway during mitoisis. GORASP2 plays a role in the assembly and membrane stacking of the Golgi cisternae, and in the process by which Golgi stacks reform after mitotic breakdown.GORASP2 is supposed to regulate the intracellular transport and presentation of a defined set of transmembrane proteins, such as transmembrane TGFA.