GOT1 Monoklonaler Antikörper

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• KD/KO Validated

GOT1 Monoklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Huhn, human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

5A12E10

Kat-Nr. : 60317-1-PBS

Synonyme

GIG18, EC:2.6.1.1, cCAT, CAT, cAspAT

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  WB analysis using 60317-1-Ig (same clone as 60317-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 60317-1-Ig (GOT1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60317-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 60317-1-Ig (same clone as 60317-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 60317-1-Ig (GOT1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60317-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 60317-1-Ig (same clone as 60317-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 60317-1-Ig (GOT1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60317-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rat heart using 60317-1-Ig (same clone as 60317-1-PBS)

  rat heart tissue were subjected to SDS PAGE followed by western blot with 60317-1-Ig (GOT1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60317-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 60317-1-Ig (same clone as 60317-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 60317-1-Ig (GOT1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60317-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 60317-1-Ig (same clone as 60317-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 60317-1-Ig (GOT1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60317-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 60317-1-Ig (same clone as 60317-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using GOT1 antibody (60317-1-Ig, Clone: 5A12E10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60317-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

60317-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA GOT1 und zeigt Reaktivität mit Huhn, human, Maus, Ratten

• Getestete Reaktivität: Huhn, human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: GOT1 fusion protein Ag7012
• Vollständiger Name: glutamic-oxaloacetic transaminase 1, soluble (aspartate aminotransferase 1)
• Berechnetes Molekulargewicht: 46 kDa
• Beobachtetes Molekulargewicht: 40-46 kDa
• GenBank-Zugangsnummer: BC000498
• Gene symbol: GOT1
• Gene ID (NCBI): 2805
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Glutamate oxaloacetate transaminase 1 (GOT1) catalyzes the reversible reaction of L-aspartate and alpha-ketoglutarate into oxaloacetate and L-glutamate and plays a key role in carbon and nitrogen metabolism. GOT1 can potentially control the intracellular levels of reactive oxygen species (ROS) through NADPH synthesis and enhances tumor growth. GOT1 expression correlates with the growth of several tumors.