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GOT1 Monoklonaler Antikörper

GOT1 Monoklonal Antikörper für IF

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

IF

Konjugation

CoraLite®594 Fluorescent Dye

CloneNo.

5A12E10

Kat-Nr. : CL594-60317

Synonyme

CAT, GIG18, GOT1, Transaminase A

Galerie der Validierungsdaten

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  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CoraLite®594-conjugated GOT1 Monoclonal antibody (CL594-60317)

    IF Staining of HepG2 using CL594-60317

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite®594 GOT1 antibody (CL594-60317, Clone: 5A12E10 ) at dilution of 1:200.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CoraLite®594-conjugated GOT1 Monoclonal antibody (CL594-60317)

    IF Staining of HepG2 using CL594-60317

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite®594 GOT1 antibody (CL594-60317, Clone: 5A12E10 ) at dilution of 1:200.

Geprüfte Anwendungen

Erfolgreiche Detektion in IFHepG2-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Immunfluoreszenz (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

CL594-60317 bindet in IF GOT1 und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Maus / IgG1
Klonalität Monoklonal
Typ Antikörper
Immunogen GOT1 fusion protein Ag7012
Vollständiger Name glutamic-oxaloacetic transaminase 1, soluble (aspartate aminotransferase 1)
Berechnetes Molekulargewicht 46 kDa
Beobachtetes Molekulargewicht 40-46 kDa
GenBank-ZugangsnummerBC000498
Gene symbol GOT1
Gene ID (NCBI) 2805
Konjugation CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Reinigungsmethode Protein-G-Reinigung
Lagerungspuffer BS mit 50% Glyzerin, 0,05% Proclin300, 0,5% BSA, pH 7,3.
LagerungsbedingungenBei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Glutamate oxaloacetate transaminase 1 (GOT1) catalyzes the reversible reaction of L-aspartate and alpha-ketoglutarate into oxaloacetate and L-glutamate and plays a key role in carbon and nitrogen metabolism. GOT1 can potentially control the intracellular levels of reactive oxygen species (ROS) through NADPH synthesis and enhances tumor growth. GOT1 expression correlates with the growth of several tumors.

Protokolle

Produktspezifische Protokolle
IF protocol for CL594 GOT1 antibody CL594-60317Protokoll herunterladen
Standard-Protokolle
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen