GP repeat Polyklonaler Antikörper

GP repeat Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen(20)

Rezensionen (1)

Kat-Nr. : 24494-1-AP

Synonyme

C9RAN, GGGCC, GP repeat, GPGPGPGP, poly GP, poly PG

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• 

  WB analysis of Recombinant protein using 24494-1-AP

  Recombinant proteins of SMARCA4 which contains GP repeat sequence were subjected to SDS PAGE followed by western blot with 24494-1-AP (GP repeat Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human cerebellum tissue using 24494-1-AP

  IHC results of GP-repeated antibody (24494-1-AP, 1:500) with human cerebellum tissue (C9ORF72 expansion carrier) by Prof. Dr. Manuela Neumann. Staining conditions: Ventana automatic staining system using Optiview DAB detection kit; pretreatment boiling for 30min in citrate buffer pH6.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Recombinant protein
• Erfolgreiche Detektion in IHC: humanes Cerebellum-Gewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:200-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 9 publications below
• IHC: See 5 publications below
• IF: See 6 publications below
• ELISA: See 1 publications below

Produktinformation

24494-1-AP bindet in WB, IHC, IF, ELISA GP repeat und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: GP repeat
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The C9orf72 "GGGGCC" repeat sequence codes five repeat peptide "GA repeat; GAGAGAGAGA", "GP repeat; GPGPGPGPG", "GR repeat; GRGRGRGRG", "AP repeat; APAPAPAPA" and "PR repeat; PRPRPRPRP". It was described previously that aggregated forms of poly-GA and poly-GP proteins do not enter the separation gel (PMID: 26374446). This antibody is used to detect the GP repeated sequence. The recombinant protein 6*His tag-SMARCA4 or GST tag-SMARCA4 which contains the sequence "GPGPGPGPG" were used to detect this antibody.

Publikationen

Species	Application	Title
human	IF	Nucleic Acids Res; High-throughput screen of 100 000 small molecules in C9ORF72 ALS neurons identifies spliceosome modulators that mobilize G4C2 repeat RNA into nuclear export and repeat associated non-canonical translation; Authors - Maartje J Luteijn
human	WB, IHC	Acta Neuropathol; Quantitative analysis and clinico-pathological correlations of different dipeptide repeat protein pathologies in C9ORF72 mutation carriers.; Authors - Ian R A Mackenzie
human	IHC	Acta Neuropathol; Increased prevalence of granulovacuolar degeneration in C9orf72 mutation.; Authors - Yuichi Riku
human	IF	Acta Neuropathol; Antisense RNA foci in the motor neurons of C9ORF72-ALS patients are associated with TDP-43 proteinopathy.; Authors - Johnathan Cooper-Knock
mouse		EMBO Rep; Glucose hypometabolism prompts RAN translation and exacerbates C9orf72-related ALS/FTD phenotypes; Authors - Andrew T Nelson
human	WB, IHC	Neuropathol Appl Neurobiol; Neurodegeneration in Frontotemporal Lobar Degeneration and Motor Neurone Disease associated with expansions in C9orf72 is linked to TDP-43 pathology and not associated with aggregated forms of dipeptide repeat proteins.; Authors - Yvonne Davidson

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Barbara (Verified Customer) (01-14-2019)

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: lysates of transgenic flies expressing 36 repeats of GGGGCC