GP73/GOLPH2 Monoklonaler Antikörper

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• KD/KO Validated

GP73/GOLPH2 Monoklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

3

CloneNo.

3D1F1

Kat-Nr. : 66331-1-Ig

Synonyme

C9orf155, Golgi marker, golgi membrane protein 1, Golgi membrane protein GP73, Golgi phosphoprotein 2, GOLM1, GOLPH2, GP73, GP73/GOLPH2

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Galerie der Validierungsdaten

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  WB analysis using 66331-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66331-1-Ig (GP73/GOLPH2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 66331-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66331-1-Ig (GP73/GOLPH2 antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 66331-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66331-1-Ig (GP73/GOLPH2 antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 66331-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using GP73/GOLPH2 antibody (66331-1-Ig, Clone: 3D1F1 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A549-Zellen, HEK-293-Zellen, HeLa-Zellen, LNCaP-Zellen
• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 3 publications below

Produktinformation

66331-1-Ig bindet in WB, IHC, IF, ELISA GP73/GOLPH2 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: GP73/GOLPH2 fusion protein Ag7207
• Vollständiger Name: golgi membrane protein 1
• Berechnetes Molekulargewicht: 45 kDa
• Beobachtetes Molekulargewicht: 73 kDa
• GenBank-Zugangsnummer: BC001740
• Gene symbol: GOLM1
• Gene ID (NCBI): 51280
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Golgi phosphoprotein 2 (GOLPH2, also known as GP73 or GOLM1) is a resident Golgi type-II membrane protein. It is predominantly expressed in the epithelial cells of many human tissues. GOLPH2 traffics through endosomes and can be secreted into the circulation. Its expression is upregulated in a number of tumors and GOLPH2 could be a promising serum marker for hepatocellular carcinoma. We got 73 kDa in western blotting due to phosphorylation.

Publikationen

Species	Application	Title
human	WB	Front Oncol; TCF11 Has a Potent Tumor-Repressing Effect Than Its Prototypic Nrf1α by Definition of Both Similar Yet Different Regulatory Profiles, With a Striking Disparity From Nrf2.; Authors - Meng Wang
human	WB	Cell Rep; Cholesterol suppresses GOLM1-dependent selective autophagy of RTKs in hepatocellular carcinoma.; Authors - Wei-Qing Shao ; KD Validated
human	WB	Microbiol Spectr; Associating bovine herpesvirus 1 envelope glycoprotein gD with activated phospho-PLC-γ1(S1248); Authors - Chang Liu