GP73/GOLPH2 Monoklonaler Antikörper

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• KD/KO Validated

GP73/GOLPH2 Monoklonal Antikörper für WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

3D1F1

Kat-Nr. : 66331-1-PBS

Synonyme

GOLM1, GOLPH2, GP73, 3D1F1, C9orf155

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  WB analysis using 66331-1-Ig (same clone as 66331-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66331-1-Ig (GP73/GOLPH2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66331-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 66331-1-Ig (same clone as 66331-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66331-1-Ig (GP73/GOLPH2 antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66331-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 66331-1-Ig (same clone as 66331-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66331-1-Ig (GP73/GOLPH2 antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66331-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 66331-1-Ig (same clone as 66331-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using GP73/GOLPH2 antibody (66331-1-Ig, Clone: 3D1F1 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 66331-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP51312-3

  Cytometric bead array standard curve of MP51312-3, GOLM1 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66331-2-PBS. Detection antibody: 66331-1-PBS. Standard:Ag7207. Range: 0.098-100 ng/mL.

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  Cytometric bead array standard curve of MP51312-4

  Cytometric bead array standard curve of MP51312-4, GOLM1 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66331-3-PBS. Detection antibody: 66331-1-PBS. Standard:Ag7207. Range: 0.098-100 ng/mL.

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Geprüfte Anwendungen

Produktinformation

66331-1-PBS bindet in WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA GP73/GOLPH2 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: GP73/GOLPH2 fusion protein Ag7207
• Vollständiger Name: golgi membrane protein 1
• Berechnetes Molekulargewicht: 45 kDa
• Beobachtetes Molekulargewicht: 73 kDa
• GenBank-Zugangsnummer: BC001740
• Gene symbol: GP73
• Gene ID (NCBI): 51280
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Golgi phosphoprotein 2 (GOLPH2, also known as GP73 or GOLM1) is a resident Golgi type-II membrane protein. It is predominantly expressed in the epithelial cells of many human tissues. GOLPH2 traffics through endosomes and can be secreted into the circulation. Its expression is upregulated in a number of tumors and GOLPH2 could be a promising serum marker for hepatocellular carcinoma. We got 73 kDa in western blotting due to phosphorylation.