GPAA1 Polyklonaler Antikörper

GPAA1 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen(4)

Kat-Nr. : 10104-1-AP

Synonyme

GAA1, Glycosylphosphatidylinositol anchor attachment 1 protein, GPAA 1, hGAA1

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  WB analysis using 10104-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10104-1-AP (GPAA1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 10104-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10104-1-AP (GPAA1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human stomach cancer using 10104-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 10104-1-AP (GPAA1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach cancer using 10104-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 10104-1-AP (GPAA1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HuH-7-Zellen, HepG2-Zellen, MCF-7-Zellen, MKN-45-Zellen, Maushirngewebe, Rattenhirngewebe
• Erfolgreiche Detektion in IHC: humanes Magenkrebsgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IHC: See 2 publications below
• IF: See 1 publications below

Produktinformation

10104-1-AP bindet in WB, IHC, IF, ELISA GPAA1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: GPAA1 fusion protein Ag0152
• Vollständiger Name: glycosylphosphatidylinositol anchor attachment protein 1 homolog (yeast)
• Berechnetes Molekulargewicht: 68 kDa
• Beobachtetes Molekulargewicht: 60-68 kDa
• GenBank-Zugangsnummer: BC004129
• Gene symbol: GPAA1
• Gene ID (NCBI): 8733
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

GPAA1(Glycosylphosphatidylinositol anchor attachment 1 protein) is also named as GAA1. It is the most conspicuously hydrophobic of the known subunits of GPIT, and it may play a key role in substrate recognition. It is predicted to be a multispanning membrane protein of 67 kDa with seven transmembrane (TM) domains, a large lumenal domain between the first two TM segments, and a cytoplasmically oriented N terminus bearing a potential ER retrieval signal in the form of a diarginine motif(PMID:12052837). It has 2 isoforms produced by alternative splicing and the full length protein has 2 glycosylation sites. This antibody is specific to GPAA1.

Publikationen

Species	Application	Title
human	IHC	Mod Pathol; Profiling the expression pattern of GPI transamidase complex subunits in human cancer.; Authors - Nagpal Jatin K JK
human	WB,IHC	Liver Res; Single-cell and machine learning approaches uncover intrinsic immune-evasion genes in the prognosis of hepatocellular carcinoma; Authors - Jiani Wang
mouse	IF	Nat Commun; CLIC and membrane wound repair pathways enable pandemic norovirus entry and infection; Authors - B Vijayalakshmi Ayyar
human	WB	Cell Rep; Targeting the GPI transamidase subunit GPAA1 abrogates the CD24 immune checkpoint in ovarian cancer; Authors - Alok K Mishra ; KD Validated

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