GPAA1 Polyklonaler Antikörper

GPAA1 Polyklonal Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 10104-1-PBS

Synonyme

GAA1, Glycosylphosphatidylinositol anchor attachment 1 protein, GPAA 1, hGAA1

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  WB analysis using 10104-1-AP (same clone as 10104-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 10104-1-AP (GPAA1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10104-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 10104-1-AP (same clone as 10104-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 10104-1-AP (GPAA1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10104-1-PBS in a different storage buffer formulation.

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  IHC staining of human stomach cancer using 10104-1-AP (same clone as 10104-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 10104-1-AP (GPAA1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10104-1-PBS in a different storage buffer formulation.

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  IHC staining of human stomach cancer using 10104-1-AP (same clone as 10104-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 10104-1-AP (GPAA1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10104-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

10104-1-PBS bindet in WB, IHC, Indirect ELISA GPAA1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: GPAA1 fusion protein Ag0152
• Vollständiger Name: glycosylphosphatidylinositol anchor attachment protein 1 homolog (yeast)
• Berechnetes Molekulargewicht: 68 kDa
• Beobachtetes Molekulargewicht: 60-68 kDa
• GenBank-Zugangsnummer: BC004129
• Gene symbol: GPAA1
• Gene ID (NCBI): 8733
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

GPAA1(Glycosylphosphatidylinositol anchor attachment 1 protein) is also named as GAA1. It is the most conspicuously hydrophobic of the known subunits of GPIT, and it may play a key role in substrate recognition. It is predicted to be a multispanning membrane protein of 67 kDa with seven transmembrane (TM) domains, a large lumenal domain between the first two TM segments, and a cytoplasmically oriented N terminus bearing a potential ER retrieval signal in the form of a diarginine motif(PMID:12052837). It has 2 isoforms produced by alternative splicing and the full length protein has 2 glycosylation sites. This antibody is specific to GPAA1.

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