GPAA1 Rekombinanter Antikörper

GPAA1 Rekombinant Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

CloneNo.

241591C4

Kat-Nr. : 84632-1-RR

Synonyme

241591C4, GAA1, Glycosylphosphatidylinositol anchor attachment 1 protein, GPAA 1, hGAA1

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  WB analysis using 84632-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84632-1-RR (GPAA1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 84632-1-RR

  Biolayer interferometry (BLl) kinetic assays of 84632-1-RR against Human GPAA1 were performed. The affinity constant is 0.141 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: MKN-45-Zellen, HeLa-Zellen, HepG2-Zellen, HuH-7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

84632-1-RR bindet in WB, ELISA GPAA1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: GPAA1 fusion protein Ag0152
• Vollständiger Name: glycosylphosphatidylinositol anchor attachment protein 1 homolog (yeast)
• Berechnetes Molekulargewicht: 68 kDa
• Beobachtetes Molekulargewicht: 60-68 kDa
• GenBank-Zugangsnummer: BC004129
• Gene symbol: GPAA1
• Gene ID (NCBI): 8733
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

GPAA1(Glycosylphosphatidylinositol anchor attachment 1 protein) is also named as GAA1. It is the most conspicuously hydrophobic of the known subunits of GPIT, and it may play a key role in substrate recognition. It is predicted to be a multispanning membrane protein of 67 kDa with seven transmembrane (TM) domains, a large lumenal domain between the first two TM segments, and a cytoplasmically oriented N terminus bearing a potential ER retrieval signal in the form of a diarginine motif(PMID:12052837).