GPAM Rekombinanter Antikörper

GPAM Rekombinant Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

CloneNo.

241144H6

Kat-Nr. : 84054-5-RR

Synonyme

GPAT-1, GPAT1, GPAT 1, Glycerol-3-phosphate acyltransferase 1, mitochondrial, EC:2.3.1.15

---

• 

  WB analysis using 84054-5-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84054-5-RR (GPAM antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 84054-5-RR

  WB result of GPAM antibody (84054-5-RR; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GPAM transfected HepG2 cells.

• 

  Affinity and Kinetic Characterization of 84054-5-RR

  Biolayer interferometry (BLl) kinetic assays of 84054-5-RR against Human GPAM were performed. The affinity constant is below 1 pM.

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mauslebergewebe, HepG2-Zellen, Rattenlebergewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

84054-5-RR bindet in WB, ELISA GPAM und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: GPAM fusion protein Ag3120
• Vollständiger Name: glycerol-3-phosphate acyltransferase, mitochondrial
• Berechnetes Molekulargewicht: 828 aa, 94 kDa
• Beobachtetes Molekulargewicht: 90-92 kDa
• GenBank-Zugangsnummer: BC030783
• Gene symbol: GPAM
• Gene ID (NCBI): 57678
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

GPAM (Glycerol-3-Phosphate Acyltransferase, Mitochondrial) is a Protein Coding gene. This gene encodes a mitochondrial enzyme which prefers saturated fatty acids as its substrate for the synthesis of glycerolipids. This metabolic pathway's first step is catalyzed by the encoded enzyme. Two forms for this enzyme exist, one in the mitochondria and one in the endoplasmic reticulum. Two alternatively spliced transcript variants have been described for this gene.Diseases associated with GPAM include Rhizomelic Chondrodysplasia Punctata, Type 2 and Congenital Generalized Lipodystrophy. GPAM can be detected as 90-92 kDa.