GPAT3 Polyklonaler Antikörper

GPAT3 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 30765-1-AP

Synonyme

AGPAT9, 1-AGPAT 9, 1-AGP acyltransferase 9, 1-acyl-sn-glycerol-3-phosphate O-acyltransferase 9, 1-acyl-sn-glycerol-3-phosphate O-acyltransferase 10

---

• 

  WB analysis using 30765-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 30765-1-AP (GPAT3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 30765-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 30765-1-AP (GPAT3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HT-1080 using 30765-1-AP

  HT-1080 cells were subjected to SDS PAGE followed by western blot with 30765-1-AP (GPAT3 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 30765-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 30765-1-AP (GPAT3 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse kidney using 30765-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 30765-1-AP (GPAT3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 30765-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 30765-1-AP (GPAT3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 30765-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 30765-1-AP (GPAT3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 30765-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 30765-1-AP (GPAT3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A549-Zellen, A375-Zellen, HeLa-Zellen, HT-1080.Zellen, Jurkat-Zellen, MKN-45-Zellen
• Erfolgreiche Detektion in IHC: Mauslebergewebe, Mausnierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

30765-1-AP bindet in WB, IHC, ELISA GPAT3 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: GPAT3 fusion protein Ag33717
• Vollständiger Name: 1-acylglycerol-3-phosphate O-acyltransferase 9
• Berechnetes Molekulargewicht: 49 kDa
• Beobachtetes Molekulargewicht: 40-45 kDa
• GenBank-Zugangsnummer: Glycerol-3-phosphate acyltransferase 3
• Gene symbol: AGPAT9
• Gene ID (NCBI): 84803
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

GPAT3, also known as AGPAT9, is a member of the lysophosphatidic acid acyltransferase protein family. GPAT3 is an enzyme that catalyzes the conversion of glycerol-3-phosphate to lysophosphatidic acid in the synthesis of triacylglycerol.