GPC1 Rekombinanter Antikörper

GPC1 Rekombinant Antikörper für Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Konjugation

Unkonjugiert

CloneNo.

240207E12

Kat-Nr. : 83318-3-PBS

Synonyme

glypican 1, FLJ38078, glypican, glypican1, Glypican-1

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  Sandwich ELISA standard curve of MP00342-2

  Sandwich ELISA standard curve of MP00342-2, human GPC1 Recombinant Matched Antibody Pair - PBS only. 83318-3-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg0737. 83318-2-PBS was HRP conjugated as the detection antibody. Range: 0.156-10 ng/mL.

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  Sample test of MP00342-2

  Serum of fourteen individual healthy human donors was measured. The GPC1 concentration of detected samples was determined to be 21.8 ng/mL with a range of 10.3 - 32.1 ng/mL.

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  Sample test of MP00342-2

  Urine of eight individual healthy human donors was measured. The GPC1 concentration of detected samples was determined to be 3.3 ng/mL with a range of 0.6 - 7.2 ng/mL.

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  Sample test of MP00342-2

  MCF-7 human breast cancer cells (5 x 10^6 cells/mL) were cultured in DMEM and 10% fetal bovine serum, 4 mM L-glutamine, 4500 mg/L glucose, 100 U/mL penicillin, and 100 μg/mL streptomycin sulfate. An aliquot of the cell culture supernatants was removed, assayed for human GPC1, and measured 13.1 ng/mL. HepG2 human hepatocellular carcinoma cells (5 x 10^6 cells/mL) were cultured in DMEM supplemented with 10% fetal bonive serum, 100 U/mL penicillin, and 100 μg/mL streptomycin sulfate. An aliquot of the cell culture supernatants was removed, assayed for human GPC1, and measured 7.1 ng/mL.

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  Sample test of MP00342-2

  The mean GPC1 concentration was determined to be 10.0 ng/mL in MDA-MB-468 cell extract based on a 3.2 mg/mL extract load and 19.7 ng/mL in placenta tissue extract based on a 1.8 mg/mL extract load.

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  Cytometric bead array standard curve of MP00342-2

  Cytometric bead array standard curve of MP00342-2, GPC1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83318-3-PBS. Detection antibody: 83318-2-PBS. Standard: Eg0737. Range: 1.25-40 ng/mL.

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Geprüfte Anwendungen

Produktinformation

83318-3-PBS bindet in Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test GPC1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: GPC1 fusion protein Eg0737
• Vollständiger Name: glypican 1
• Berechnetes Molekulargewicht: 558 aa, 62 kDa
• GenBank-Zugangsnummer: BC051279
• Gene symbol: GPC1
• Gene ID (NCBI): 2817
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.