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GPC1 Rekombinanter Antikörper

GPC1 Rekombinant Antikörper für WB, Cytometric bead array, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, Cytometric bead array, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

240207G5

Kat-Nr. : 83318-4-PBS

Synonyme

glypican 1, 240207G5, FLJ38078, glypican, glypican1

Filter:
  • Western Blot (WB) analysis of HepG2 cells using GPC1 Recombinant antibody (83318-4-RR)

    WB analysis of HepG2 using 83318-4-RR (same clone as 83318-4-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 83318-4-RR (GPC1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83318-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83318-4-RR

    Affinity and Kinetic Characterization of 83318-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83318-4-RR against Human GPC1 were performed. The affinity constant is 3.58 nM.

Geprüfte Anwendungen

Produktinformation

83318-4-PBS bindet in WB, Cytometric bead array, Indirect ELISA GPC1 und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Kaninchen / IgG
Klonalität Rekombinant
Typ Antikörper
Immunogen GPC1 fusion protein Eg0737
Vollständiger Name glypican 1
Berechnetes Molekulargewicht 558 aa, 62 kDa
Beobachtetes Molekulargewicht 61-65 kDa
GenBank-ZugangsnummerBC051279
Gene symbol GPC1
Gene ID (NCBI) 2817
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Glypicans are a family of HSPGs that are attached to the cell membrane by a glycosyl-phosphatidylinositol anchor. Glypicans are considered to have the ability to modulate the activities of HBGFs. The expression of glypicans is regulated temporally and spatially during development, suggesting they are involved in development and morphogenesis. To date, six glypicans (GPC1 to GPC6) have been identified in mammals. GPC1 is most ubiquitously expressed in adult tissues. GPC1 promotes efficient signaling by HBGFs and plays a critical role in cell growth and differentiation. GPC1 is involved in tumorigenesis and angiogenesis, and is frequently overexpressed in several types of tumors including pancreatic carcinoma, breast cancer and glioma.