GPC1 Rekombinanter Antikörper

GPC1 Rekombinant Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

CloneNo.

240207G5

Kat-Nr. : 83318-4-RR

Synonyme

glypican 1, Glypican-1, glypican1, glypican, FLJ38078

---

• 

  WB analysis of HepG2 using 83318-4-RR

  HepG2 cells were subjected to SDS PAGE followed by western blot with 83318-4-RR (GPC1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  Affinity and Kinetic Characterization of 83318-4-RR

  Biolayer interferometry (BLl) kinetic assays of 83318-4-RR against Human GPC1 were performed. The affinity constant is 3.58 nM.

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

83318-4-RR bindet in WB, ELISA GPC1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Fusionsprotein
• Vollständiger Name: glypican 1
• Berechnetes Molekulargewicht: 558 aa, 62 kDa
• Beobachtetes Molekulargewicht: 61-65 kDa
• GenBank-Zugangsnummer: BC051279
• Gene symbol: GPC1
• Gene ID (NCBI): 2817
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Glypicans are a family of HSPGs that are attached to the cell membrane by a glycosyl-phosphatidylinositol anchor. Glypicans are considered to have the ability to modulate the activities of HBGFs. The expression of glypicans is regulated temporally and spatially during development, suggesting they are involved in development and morphogenesis. To date, six glypicans (GPC1 to GPC6) have been identified in mammals. GPC1 is most ubiquitously expressed in adult tissues. GPC1 promotes efficient signaling by HBGFs and plays a critical role in cell growth and differentiation. GPC1 is involved in tumorigenesis and angiogenesis, and is frequently overexpressed in several types of tumors including pancreatic carcinoma, breast cancer and glioma.