Glypican 2 Rekombinanter Antikörper

Glypican 2 Rekombinant Antikörper für Cytometric bead array, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

Cytometric bead array, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

230518A4

Kat-Nr. : 83241-1-PBS

Synonyme

GPC2, 230518A4, FLJ38962, Secreted glypican-2

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  FC experiment of HeLa using 83241-1-RR (same clone as 83241-1-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug Gpc2 Recombinant Antibody (83241-1-RR, Clone:230518A4) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83241-1-PBS in a different storage buffer formulation.

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  FC experiment of SH-SY5Y using 83241-1-RR (same clone as 83241-1-PBS)

  1x10^6 SH-SY5Y cells were intracellularly stained with 0.25 ug Gpc2 Recombinant Antibody (83241-1-RR, Clone:230518A4) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83241-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP00156-2

  Cytometric bead array standard curve of MP00156-2, Glypican 2 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83241-1-PBS. Detection antibody: 83241-2-PBS. Standard: Ag4041. Range: 2.5-80 ng/mL.

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  Affinity and Kinetic Characterization of 83241-1-RR

  Biolayer interferometry (BLl) kinetic assays of 83241-1-RR against Human Glypican 2 were performed. The affinity constant is below 1 pM.

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Geprüfte Anwendungen

Produktinformation

83241-1-PBS bindet in Cytometric bead array, Indirect ELISA Glypican 2 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Glypican 2 fusion protein Ag4041
• Vollständiger Name: glypican 2
• Berechnetes Molekulargewicht: 579 aa, 63 kDa
• Beobachtetes Molekulargewicht: 62，70-100 kDa
• GenBank-Zugangsnummer: BC027972
• Gene symbol: GPC2
• Gene ID (NCBI): 221914
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

GPC2 (glypican 2), which is expected to be located in cell membrane and extracellular space. It is expressed in lymphoid tissue, skin and testis. The calculated molecular weight of the protein is 62 kDa. GPC2 is mainly active in growing nervous tissues and thyroid cancer tissues (PMID: 28616017). It participates in the growth and differentiation of neuronal axons. Increasing evidence has demonstrated the overexpression of GPC2 in neuroblastoma, a kind of childhood cancer. There is a discovery that GPC2 can be employed as a diagnostic, prognostic, and immunological predictor of generalized cancers. The study may broaden the train of thought toward application of GPC2 in immunotherapy (PMID: 35345673).